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Recombinant Human Lefty-A Protein

Catalog #: 746-LF
11 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
746-LF-025/CF

With Carrier

Catalog # Availability Size / Price Qty
746-LF-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (11)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Lefty-A Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce cell death using Mv1Lu mink lung epithelial cells. The ED50 for this effect is 0.5-3 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human Lefty-A protein
    Mouse Lefty-1 Propeptide
    (Leu22-Arg77)
Accession # Q64280		 HHHHHH
 Human Lefty-A
(Phe78-Pro366) 
Accession # O00292       
N-terminus
C-terminus
Accession #
Q64280
N-terminal Sequence
Analysis
Leu22
Predicted Molecular Mass
39 kDa
SDS-PAGE
44 kDa, 38 kDa and 34 kDa, reducing conditions

Product Datasheets

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746-LF (with carrier)

Product Datasheet
COA
SDS

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746-LF/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

746-LF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

746-LF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lefty-A

Lefty was first identified in a screen for undifferentiated cell-specific cDNAs from the P19 mouse embryonal carcinoma cells. Its mRNA expression on the left side of the developing embryo earned the name “Lefty”. The human orthologue was initially identified as Ebaf, Endometrial Bleeding-Associated Factor. Two genes exist in mouse (Lefty-1 and Lefty-2) and two in humans (Lefty-A and Lefty-B). By amino acid sequence, human Lefty-A and -B are more similar to each other (96%) than to either Lefty-1 or -2 in the mouse (81 - 82% identical). Lefty contains the six cysteine residues that are conserved among TGF-beta related proteins and that are necessary to form the cysteine-knot structure. However, Lefty is distinct from other family members in that it has two RXXR cleavage sites, a longer carboxy terminal sequence, and it lacks the cysteine residue required for intermolecular disulfide linkage. Thus, mature forms of Lefty are larger than mature forms of other TGF-beta -related proteins.

Lefty homologues have been identified in other vertebrate organisms including chick, frog, and zebrafish. Although the amino acid sequence identity is not well conserved among vertebrate species, the expression pattern of Lefty on the left side is well conserved. Furthermore its function in patterning left-right asymmetry of the developing organ systems such as the heart and lung is consistent in all vertebrate species examined. Lefty acts as an antagonist to Nodal signaling, potentially by competing for binding to a common receptor.

References
  1. Meno, C. et al. (1996) Nature 381:151.
  2. Kosaki, K. et al. (1999) Am. J. Hum. Genet. 64:712.
  3. Schier, A.F. and M.M. Shen, (1999) Nature 403:385.
  4. Branford, W.W. et al. (2000) Dev. Biol. 223:291.
  5. Tabibzadeh, S. et al. (2000) J. Clin. Endocr. Metab. 85:2526.
  6. Ulloa, L. et al. (2001) J. Biol. Chem. 276:21387.
Entrez Gene IDs
7044 (Human)
Alternate Names
EBAFTGF-beta-4; endometrial bleeding associated factor; Endometrial bleeding-associated factor; Lefta; LEFTAProtein lefty-A; left-right determination factor 2; Left-right determination factor A; LEFTY2; LeftyA; Lefty-A; LEFTYATransforming growth factor beta-4; MGC46222; Protein lefty-2; TGFB4endometrial bleeding associated factor (left-right determination, factor A;transforming growth factor beta superfamily)

Citations for Recombinant Human Lefty-A Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. LEFTY2/endometrial�bleeding-associated factor up-regulates Na+�Coupled Glucose Transporter SGLT1 expression and Glycogen Accumulation in Endometrial Cancer Cells
    Authors: N Zeng, T Okumura, M Alauddin, S Khozooei, J Rajaxavier, S Zhang, Y Singh, B Shi, SY Brucker, D Wallwiener, S Takeda, F Lang, MS Salker
    PLoS ONE, 2020-04-01;15(4):e0230044.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. PMN-MDSCs Enhance CTC Metastatic Properties through Reciprocal Interactions via ROS/Notch/Nodal Signaling
    Authors: ML Sprouse, T Welte, D Boral, HN Liu, W Yin, M Vishnoi, D Goswami-Se, L Li, G Pei, P Jia, IC Glitza-Oli, D Marchetti
    Int J Mol Sci, 2019-04-18;20(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Nodal Signaling Regulates Germ Cell Development and Establishment of Seminiferous Cords in the Human Fetal Testis
    Authors: A Jørgensen, J Macdonald, JE Nielsen, KR Kilcoyne, S Perlman, L Lundvall, L Langhoff T, K Juul Hare, H Frederikse, AM Andersson, NE Skakkebæk, A Juul, RM Sharpe, E Rajpert-De, RT Mitchell
    Cell Rep, 2018-11-13;25(7):1924-1937.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Novel regulatory mechanisms for the SoxC transcriptional network required for visual pathway development
    Authors: KC Chang, J Hertz, X Zhang, XL Jin, P Shaw, BA Derosa, JY Li, P Venugopala, DA Valenzuela, RD Patel, KR Russano, SA Alshamekh, C Sun, K Tenerelli, C Li, D Velmeshev, Y Cheng, TM Boyce, A Dreyfuss, MS Uddin, KJ Muller, DM Dykxhoorn, JL Goldberg
    J. Neurosci., 2017-04-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. LEFTYA Activates the Epithelial Na+ Channel (ENaC) in Endometrial Cells via Serum and Glucocorticoid Inducible Kinase SGK1
    Cell Physiol Biochem, 2016-09-08;39(4):1295-306.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. LeftyA decreases Actin Polymerization and Stiffness in Human Endometrial Cancer Cells.
    Authors: Madhuri S Salker, Nicolas Schierbau, Nour Alowayed, Yogesh Singh, Andreas F Mack, Christos Stournara, Tilman E Schäffer, Florian Lang
    Scientific Reports, 2016-07-11;0(0):2045-2322.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Role of Lefty in the anti tumor activity of human adult liver stem cells.
    Authors: Cavallari, C, Fonsato, V, Herrera, M B, Bruno, S, Tetta, C, Camussi, G
    Oncogene, 2012-04-02;32(7):819-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Deregulation of the serum- and glucocorticoid-inducible kinase SGK1 in the endometrium causes reproductive failure.
    Authors: Salker MS, Christian M, Steel JH, Nautiyal J, Lavery S, Trew G, Webster Z, Al-Sabbagh M, Puchchakayala G, Foller M, Landles C, Sharkey AM, Quenby S, Aplin JD, Regan L, Lang F, Brosens JJ
    Nat. Med., 2011-10-16;17(11):1509-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed.
    Authors: McLean AB, D&amp;apos;Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S
    Stem Cells, 2007-01-01;25(1):29-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Immunophenotypic clustering of myelodysplastic syndromes.
    Authors: Maynadie M, Picard F, Husson B, Chatelain B, Cornet Y, Le Roux G, Campos L, Dromelet A, Lepelley P, Jouault H, Imbert M, Rosenwadj M, Verge V, Bissieres P, Raphael M, Béné MC, Feuillard J, Feuillard J
    Blood, 2002-10-01;100(7):2349-56.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay

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