Recombinant Human LH alpha/beta Heterodimer Protein
Recombinant Human LH alpha/beta Heterodimer Protein Summary
Product Specifications
Ala25-Ser116 ( alpha chain) & Ser21-Leu141 ( beta chain)
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8899-LH
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
8899-LH/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in sterile PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: LH alpha/beta Heterodimer
Luteinizing Hormone (LH) is a 42 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta chains. The alpha subunit (CG alpha ) is also a component of Follicle-Stimulating Hormone (FSH), Thyroid-Stimulating Hormone, and Chorionic Gonadotropin. The unique beta subunit confers the protein’s specific biological action and is responsible for the interaction with its receptor (1, 2). The approximately 20 kDa human CG alpha subunit shares 73% and 72% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. The approximately 18 kDa human LH beta subunit shares 71% and 72% aa sequence identity with the mouse and rat orthologs, respectively. Multiple isoforms of LH exist due to differences in the post-translational glycosylation, sialylation, and sulphation modifications of its subunits (3-6). The composition, longevity, and activity of the different LH isoforms vary throughout a woman’s menstrual cycle and reproductive life cycle (7). LH is produced and secreted by the anterior pituitary gland. Its secretion is controlled by Gonadotropin-Releasing Hormone from the hypothalamus; however, LH secretion can also be stimulated by estradiol (7, 8). LH works in concert with FSH to regulate female reproduction; FSH stimulates follicular growth and LH induces ovulation (9). LH also drives formation of the corpus luteum by promoting progesterone production (7). Additionally, LH has been suggested to stimulate the adrenal gland in postmenopausal women to induce secretion of sulfated DHEA, a precursor to androgens (10, 11). In the testis, LH induces Leydig cell production of testosterone (7). Hypersecretion of LH has been shown to occur in women with polycystic ovary syndrome and is associated with an increased risk of infertility and miscarriage (12, 13). Additionally, increased serum LH levels are associated with decreased cognition and have been implicated in the development and progression of Alzheimer’s disease (14, 15).
- Keutmann, H.T. et al. (1987) Proc. Natl. Acad. Sci. USA 84:2038.
- Keutmann, H.T. et al. (1992) Mol. Endocrinol. 6:904.
- Wilson, C.A. et al. (1990) J. Endocrinol. 125:3.
- Stanton, P.G. et al. (1993) J. Endocrinol. 138:529.
- Stanton, P.G. et al. (1996) Mol. Cell. Endocrinol. 125:133.
- Bergendah, M. and J.D. Veldhuis (2001) Hum. Reprod. 16:1058.
- Choi, J. and J. Smitz (2014) Mol. Cell. Endocrinol. 383:203.
- Marshall, J.C. et al. (1993) Trans. Am. Clin. Climatol. Assoc. 104:31.
- Raju, G.A. et al. (2013) J. Hum. Reprod. Sci. 6:227.
- Alevizaki, M. et al. (2006) Eur. J. Endocrinol. 154:875.
- Moran, F.M. et al. (2013) Menopause 20:329.
- Balen, A.H. (1995) J. R. Soc. Med. 88:339P.
- Blank, S.K. et al. (2006) Hum. Reprod. Update 12:351.
- Webber, K.M. et al. (2007) Clin. Med. Res. 5:177.
- Blair, J.A. et al. (2015) Front. Endocrinol. (Lausanne) 6:45.
Citations for Recombinant Human LH alpha/beta Heterodimer Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Regulation of corticosteroid-binding globulin release in murine leydig tumor cell line mLTC-1 by luteinizing hormone and interleukin-6
Authors: Zhang, Y;Liu, L;Yang, C;Xie, W;Wang, J;
Archives of biochemistry and biophysics
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
A novel potential role of pituitary gonadotropins in the pathogenesis of human colorectal cancer
Authors: W Marlicz, A Poniewiers, S Rzeszotek, R Bartoszews, K Skonieczna, T Starzy?ska, MZ Ratajczak
PLoS ONE, 2018-03-01;13(3):e0189337.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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