Recombinant Human Lymphotoxin-alpha/TNF-beta Summary
Product Specifications
Leu35-Leu205, with an N-terminal Met
Accession # P01374
Analysis
Product Datasheets
211-TB/CF (carrier free)
Discontinued Product
211-TB
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Lymphotoxin-alpha/TNF-beta
Tumor necrosis factor beta (TNF-beta ), also known as lymphotoxin-alpha (LT-alpha ), and TNF-alpha, are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical, effects. Among these effects is the ability to kill certain tumor cells directly, from which the names tumor necrosis factor and lymphotoxin both derive. Mature TNF‑ beta /LT‑ alpha and TNF-alpha share approximately 35% protein sequence homology and the biologically active secreted forms of both proteins are homotrimers. Whereas TNF-alpha can exist as a type II membrane protein, TNF-beta /LT-alpha possesses a typical signal peptide sequence and is a secreted protein. It has been shown that TNF‑ beta /LT‑ alpha is also present on the cell surface of activated T, B and LAK cells as a heteromeric complex with LT-beta, a type II membrane protein that is another member of the TNF ligand family. The genes for TNF-alpha, TNF‑ beta /LT‑ alpha, and LT‑ beta are closely linked within the major histocompatibility complex.
TNF-beta /LT-alpha is expressed in activated T- and B‑lymphocytes. In addition to its cytotoxic action on tumor cells, TNF-beta /LT-alpha has been shown to be a mediator of inflammation and immune function. Evidence is also accumulating that TNF-beta /LT-alpha and TNF-alpha are mediators in the pathogenesis of certain autoimmune diseases. TNF-beta /LT-alpha has also been shown to have a role in lymphoid organ development. Human and mouse TNF-beta /LT-alpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity.
Citations for Recombinant Human Lymphotoxin-alpha/TNF-beta
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Clostridial C3 Toxins Enter and Intoxicate Human Dendritic Cells
Authors: M Fellermann, C Huchler, L Fechter, T Kolb, F Wondany, D Mayer, J Michaelis, S Stenger, K Mellert, P Möller, TFE Barth, S Fischer, H Barth
Toxins (Basel), 2020-09-01;12(9):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
EphA2 contributes to disruption of the blood-brain barrier in cerebral malaria
Authors: TK Darling, PN Mimche, C Bray, B Umaru, LM Brady, C Stone, CE Eboumbou M, TE Lane, LS Ayong, TJ Lamb
PLoS Pathog., 2020-01-30;16(1):e1008261.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
BTKCys481Serdrives ibrutinib resistance via ERK1/2, and protects BTKWild-TypeMYD88 mutated cells by a paracrine mechanism
Authors: JG Chen, X Liu, M Munshi, L Xu, N Tsakmaklis, MG Demos, A Kofides, ML Guerrera, GG Chan, CJ Patterson, KE Meid, J Gustine, T Dubeau, P Severns, JJ Castillo, ZR Hunter, J Wang, SJ Buhrlage, NS Gray, SP Treon, G Yang
Blood, 2018-03-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Dendritic cell regulation of NK-cell responses involves lymphotoxin-alpha, IL-12, and TGF-beta.
Authors: Sarhan D, Palma M, Mao Y, Adamson L, Kiessling R, Mellstedt H, Osterborg A, Lundqvist A
Eur J Immunol, 2015-04-17;45(6):1783-93.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1.
Authors: Steeland S, Puimege L, Vandenbroucke R, Van Hauwermeiren F, Haustraete J, Devoogdt N, Hulpiau P, Leroux-Roels G, Laukens D, Meuleman P, De Vos M, Libert C
J Biol Chem, 2014-12-23;290(7):4022-37.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Inhibition of activin A ameliorates skeletal muscle injury and rescues contractile properties by inducing efficient remodeling in female mice.
Authors: Yaden B, Wang Y, Wilson J, Culver A, Milner A, Datta-Mannan A, Shetler P, Croy J, Dai G, Krishnan V
Am J Pathol, 2014-04-01;184(4):1152-66.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Regulatory T-cells and cAMP suppress effector T-cells independently of PKA-CREM/ICER: a potential role for Epac.
Authors: Vang, Amanda G, Housley, William, Dong, Hongli, Basole, Chaitali, Ben-Sasson, Shlomo Z, Kream, Barbara, Epstein, Paul M, Clark, Robert B, Brocke, Stefan
Biochem J, 2013-12-15;456(3):463-73.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
J. Exp. Med., 2006-11-20;203(12):2763-77.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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Average Rating: 4 (Based on 1 Review)
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Reason for Rating: Two peaks are observed to elute off the SEC. The first peak elutes between the 17kDa & 44kDa MW standards. The second peak elutes between the 1.3kDa & 17kDa MW standards. The MW of this homotrimer is 56376Da and should run after the 44kDa MW standard.
