Recombinant Human MARCO Protein, CF

Catalog #: 7586-MA Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7586-MA-050

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Recombinant Human MARCO Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles. Sankala, M. et al. (2002) J. Biol. Chem. 277:33378. The ED₅₀ for this effect is 0.3-1.5 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human MARCO protein
Met79-Val520, with an N-terminal 6-His tag

Accession #

Q9UEW3

N-terminal Sequence
Analysis

His

Predicted Molecular Mass

44.7 kDa

SDS-PAGE

60-70 kDa, reducing conditions

Product Datasheets

7586-MA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7586-MA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: MARCO

MARCO (macrophage receptor with collagenous structure), also known as SCARA2, is an 80 kDa type II transmembrane glycoprotein in the class A scavenger receptor family (1). Human MARCO consists of a 43 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 456 aa extracellular domain (ECD) that includes a stalk region, a collagen‑like region, and one SRCR domain (2). Within the ECD, human MARCO shares 69% aa sequence identity with mouse and rat MARCO. MARCO is constitutively expressed on the surface of splenic and lymph node macrophages (3). Its expression can be induced on Kupffer cells, alveolar macrophages, and glial cells by microbial infection, chemical irritants, and Th1 polarizing factors (4‑6). The SRCR domain mediates binding of MARCO to its various ligands, while the collagen‑like region mediates assembly into a disulfide-linked trimer (2, 3, 7). MARCO binds bacterial LPS and lipoteichoic acid, modified LDL, CpG oligonucleotides, UGRP1, silica, and TiO₂ (3, 7‑10). It interacts in cis with the formyl peptide receptors FPR1 and FPRL1 on astrocytes and microglia (11). MARCO ligation promotes the production of inflammatory mediators by macrophages (8). MARCO mediated internalization of some ligands prevents their activation of cell surface TLR4 but enables their activation of intracellular TLR3 (12). MARCO contributes to the clearance of apoptotic cells and inhaled bacteria, mast cell mediated silicosis, and the amelioration of allergen or ozone induced lung inflammation (4, 13‑16). It is required for the organization of the splenic marginal zone and the interaction of splenic macrophages and B cells (17).

References