Recombinant Human MBL Protein, CF Summary
Product Specifications
Glu21-Ile248
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9086-MB
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Mannan is coated at 0.1 µg/mL (100 µL/well), Recombinant Human MBL (Catalog # 9086-MB) binds with a typical ED50 of 25-150 ng/mL.
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Background: MBL
Human mannose/mannan-binding lectin (MBL) is a 25-30 kDa secreted glycoprotein in the collectin family of pattern-recognition molecules (1, 2). Mature human MBL contains a cysteine-rich region, a collagen-like segment, and a C-type lectin domain that binds to neutral bacterial carbohydrates (3). Human MBL shares 61% amino acid sequence identity with mouse and rat MBL and carries variable post-translation modifications including O-glycosylation and proline and lysine hydroxylation (4). Its collagen-like region mediates MBL association into disulfide-stabilized trimers which further associate into complexes containing three or four copies of the basic trimer (4, 5). MBL is secreted by hepatocytes and opsonizes bacteria through interactions with microbial carbohydrates (5). Tetrameric complexes of MBL show greater carbohydrate binding capacity compared to the trimers (5). MBL multimers can associate with the serine proteases MASP-1, -2, and -3 and promote their cleavage of Complement Component C3 (5-8). Proteolytic cleavage of C3 triggers activation of the complement system and formation of the membrane attack complex, leading to destruction of opsonized bacteria (2). In addition, MBL binds to the scavenger receptor CD91 which mediates the clearance of apoptotic material (9).
- Scorza, M. et al. (2015) Clin. Chim. Acta 451:78.
- Matsushita, M. et al. (2013) Arch. Immunol. Ther. Exp. (Warsz.) 61:273.
- Ezekowitz, R.A.B. et al. (1988) J. Exp. Med. 167:1034.
- Jensen, P.H. et al. (2005) J. Biol. Chem. 280:11043.
- Teillet, F. et al. (2005) J. Immunol. 174:2870.
- Moller-Kristensen, M. et al. (2007) Int. Immunol. 19:141.
- Thiel, S. et al. (2000) J. Immunol. 165:878.
- Vorup-Jensen, T. et al. (2000) J. Immunol. 165:2093.
- Duus, K. et al. (2010) FEBS J. 277:4956.
Citations for Recombinant Human MBL Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Fasciola hepatica is refractory to complement killing by preventing attachment of mannose binding lectin (MBL) and inhibiting MBL-associated serine proteases (MASPs) with serpins
Authors: C De Marco V, HL Jewhurst, J Dobó, P Gál, JP Dalton, K Cwiklinski
PloS Pathogens, 2022-01-10;18(1):e1010226.
Species: Fasciola hepatica
Sample Types: Whole Organism
Applications: Bioassay -
Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma
Authors: J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani
PLoS ONE, 2019-07-25;14(7):e0219480.
Species: Human
Sample Types: Natural Protein
Applications: Bioassay -
The role of Mannose Binding Lectin in the immune response against Borrelia burgdorferi sensu lato
Authors: J Coumou, A Wagemakers, S Narasimhan, TJ Schuijt, JI Ersoz, A Oei, OJ de Boer, JJTH Roelofs, E Fikrig, JW Hovius
Sci Rep, 2019-02-05;9(1):1431.
Species: Parasite - Borrelia burgdorferi
Sample Types: Whole Cells
Applications: Bioassay -
Highly modified and immunoactive N-glycans of the canine heartworm
Authors: F Martini, B Eckmair, S Štefani?, C Jin, M Garg, S Yan, C Jiménez-Ca, A Hykollari, C Neupert, L Venco, D Varón Silv, IBH Wilson, K Paschinger
Nat Commun, 2019-01-08;10(1):75.
Applications: Binding Assay -
Mannose-Binding Lectin: A Potential Therapeutic Candidate against Candida Infection
Authors: NM Hammad, NE El Badawy, HA Ghramh, LM Al Kady
Biomed Res Int, 2018-05-02;2018(0):2813737.
Species: Yeast
Sample Types: Whole Cells
Applications: Bioassay -
Ross River virus envelope glycans contribute to disease through activation of the host complement system
Authors: BM Gunn, JE Jones, RS Shabman, AC Whitmore, S Sarkar, LK Blevins, TE Morrison, MT Heise
Virology, 2018-01-08;515(0):250-260.
Species: Hamster
Sample Types: Whole Cells
Applications: Bioassay -
Expression of Lewis-a glycans on polymorphonuclear leukocytes augments function by increasing transmigration
Authors: JC Brazil, R Sumagin, SR Stowell, G Lee, NA Louis, RD Cummings, CA Parkos
J. Leukoc. Biol., 2017-06-09;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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