Recombinant Human MDGA1 Fc Chimera Protein, CF

Catalog #: 11368-MD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11368-MD-050

Recombinant Human MDGA1 Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human MDGA1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human MDGA1 Fc Chimera (Catalog # 11368-MD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Neuroligin 2/NLGN2 (Catalog # 5645-NL) binds with an ED₅₀ of 0.125-1.50 µg/mL.

Source

Human embryonic kidney cell, HEK293-derived human MDGA1 protein

Human MDGA1 (Gln19-Gly931) Accession # Q8NFP4.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q8NFP4.1

N-terminal Sequence
Analysis

No results obtained: Gln19 predicted

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

128 kDa

SDS-PAGE

125-145 kDa, under reducing conditions.

Product Datasheets

11368-MD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11368-MD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human MDGA1 Fc Chimera Protein (Catalog # 11368-MD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Neuroligin 2/NLGN2 (5645-NL) binds with an ED₅₀ of 0.125-1.50 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human MDGA1 Fc Chimera Protein (Catalog # 11368-MD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 125-145 kDa and 250-290 kDa, respectively.

Reconstitution Calculator

Background: MDGA1

MDGA1 (MAM domain containing glycosylphosphatidylinositol anchor 1) is a 135‑140 kDa glycoprotein within the IgCAM superfamily of adhesion molecules (1‑3). MDGA1 is thought to mediate neuronal migration, neurite outgrowth, and cell-cell adhesion within the central and peripheral nervous system (1, 4‑8). Human MDGA1 precursor is a 955 amino acid (aa) protein that produces a 914 aa mature protein with six Ig-like domains, a fibronectin type III domain (aa 640-739), a MAM domain (aa 751-918), and a GPI anchor (aa 932) (1-3). Mature human MDGA1 shares 95%, 96%, and 98% aa sequence identity with mature mouse, rat, and canine MDGA1, respectively. Potential human variants of 973, 904 and 587 aa contain a 48 aa substitution for aa 936-955, a 58 aa substitution for aa 847-955, and a 51 aa substitution for aa 537-955, respectively. Human MDGA1 also shares 54% aa sequence identity with human MDGA2, and may also share some functional redundancy (1, 4). MDGA1 is mainly expressed on restricted populations of neurons in the central and peripheral nervous system, such as embryonic neurons destined for cortical layers 2/3, migrating basilar pontine neurons and D1 interneurons of the spinal cord (1, 5‑7). Deletion or down‑regulation of mouse MDGA1 slows radial migration of neurons, indicating a role for MDGA1 in radial migration of cortical neurons (4, 5). MAM and Ig-like domains are involved in heterophilic and homophilic adhesion (7, 8). MDGA1 expression is also reported on primary cells and cell lines from leukemias, lymphomas, and tumors of the lung, colon, uterus, stomach and breast (3).

References