Recombinant Human mGluR3 Protein, CF

Catalog #: 9527-UR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9527-UR-100

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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human mGluR3 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Chinese Hamster Ovary cell line, CHO-derived human mGluR3 protein
Asp25-Ser507, with a C-terminal 6-His tag

Accession #

Q14832-1

N-terminal Sequence
Analysis

Asp25

Structure / Form

Disulfide linked homodimer

Predicted Molecular Mass

56 kDa

SDS-PAGE

60-74 kDa, reducing conditions

Product Datasheets

9527-UR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9527-UR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: mGluR3

Metabotropic glutamate receptors (mGluRs) are coupled to effector systems through GTP-binding proteins and modulate glutamate neurotransmission in the central and peripheral nervous systems (1). Structurally, members from this family are characterized by a large N-terminal extracellular domain (ECD), seven transmembrane, and a cytoplasmic carboxyl-terminal domain variable in length. Two ECDs dimerize together and large conformational changes are induced when agonists bind to one or both domains (3). Intracellularly, a short C-terminus interacts directly with a G-protein (2). The receptors are subdivided into three groups (I-III) based on sequence homology, signal transduction and pharmacological properties (1). The Group II receptors comprise mGluR2 and mGluR3, which share high sequence homology. mGluR3 is a presynaptic receptor expressed on both neurons and glia, and its activation regulates cAMP accumulation (1). Mature human mGluR3 is 857 amino acids (aa) in length and aa 2-507 represents the N-terminal ECD (4), with the apparent molecular mass approximately 66 kDa in SDS-PAGE under reducing conditions. Over aa 2-507, human mGluR3 shares 97% aa sequence identity with mouse and rat mGluR3.

References

Conn PJ, et al. (1997) Annu Rev Pharmacol Toxicol 37:205.
Pin JP, et al. (1995) Neuropharmacology. 34:1.
Niswender CM, et al. (2010) Annu Rev Pharmacol Toxicol 50:295.
Monn, J.A. et al. (2015) J Med Chem 58:7526.

Long Name

Metabotropic Glutamate Receptor 3

Entrez Gene IDs

2913 (Human); 108069 (Mouse); 24416 (Rat)

Alternate Names

GLUR3; glutamate receptor, metabotropic 3; GPRC1C; GPRC1Cglutamate metabotropic receptor 3; GRM3; mGlu3; mGluR3; MGLUR3metabotropic glutamate receptor 3