Recombinant Human mGluR5 Protein, CF

Catalog #: 9529-UR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9529-UR-100

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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human mGluR5 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Mouse myeloma cell line, NS0-derived human mGluR5 protein
Ser19-Ser509, with a C-terminal 6-His tag

Accession #

P41594

N-terminal Sequence
Analysis

Ser19

Structure / Form

Disuflide linked homodimer

Predicted Molecular Mass

56 kDa

SDS-PAGE

61-71 kDa, reducing conditions

Product Datasheets

9529-UR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9529-UR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: mGluR5

Metabotropic glutamate receptors (mGluRs) are coupled to effector systems through GTP-binding proteins and modulate glutamate neurotransmission in the central and peripheral nervous systems (1). Structurally, members from this family are characterized by a large N-terminal extracellular domain (ECD), seven transmembrane, and a cytoplasmic carboxyl-terminal domain variable in length. Two ECDs dimerize together and large conformational changes are induced when agonists bind to one or both domains (3). Intracellularly, a short C-terminus interacts directly with a G-protein (2). The receptors are subdivided into three groups (I-III) based on sequence homology, signal transduction and pharmacological properties (1). Group I includes mGluR1 and mGluR5. This group has been shown to activate phospholipase C and regulates neuronal ion channel activity. Human mGluR5 is 1212 amino acids (aa) in length. The 2-509 amino acids domain represents the ECD, with the apparent molecular mass approximately 64 kDa in SDS-PAGE under reducing conditions. Through its ECD, mGluR5 either homodimerizes or heterodimerizes with the Ca⁺⁺ sensor receptor (4). Over aa 2-509, human mGluR5 is 98% aa identical to mouse and rat mGluR5.

References