Recombinant Human MICA His-tag Protein, CF Summary
Product Specifications
Ala23-Gln308, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10153-MA
| Formulation | Lyophilized from a 0.2 μm filtered solution in MOPS, NaCL with trehalose. |
| Reconstitution | Reconstitute at 250 μg/mL in sterile water |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human NKG2D Fc Chimera (Catalog # 1299-NK) is immobilized at 1.0 µg/mL, 100 µL/well, the concentration of Recombinant MICA His-tag (Catalog # 10153-MA) that produces 50% of the optimal binding response is approximately 0.1-0.5 µg/mL.
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2 μg/lane of Recombinant Human MICA His-tag (Catalog # 10153-MA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa.
Reconstitution Calculator
Background: MICA
Major Histocompatibility Complex Class I Chain-related Gene A (MICA) is a transmembrane glycoprotein that functions as a ligand for human Natural-Killer Group 2 Member D (NKG2D) (1). MICA, along with MICB, which shares 91% sequence identity (2), are members of the non-classical MHC class I family. The domain structure of MICA is similar to classical MHC class I proteins: three extracellular Ig-like domains ( alpha 1, alpha 2, and alpha 3) in the extracellular domain (ECD), a transmembrane segment, and a carboxy-terminal cytoplasmic tail (2). Despite structural similarities, MICA shares only 27% amino acid (aa) identity with human MHC class I proteins (3). Both MICA and MICB display a significant degree of polymorphism within the ECD and these allow MICA to be reconfigured and bind with NKG2D rather than binding with beta 2-microglobulin (4, 5). MICA genes occur in most mammalian species, but are absent from mouse and rat (2, 4). The genes encoding MICA and MICB are found within the MHC on the short arm of human chromosome 6 (2, 3). MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells (6). Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells (4, 7). MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases (8).
- Huang, C. et al. (2018) Sci. Rep. 8:15821.
- Bahram, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91:6259.
- Cosman, D. et al. (2001) Immunity 14:123.
- Stephens, H. A. F. et al. (2001) Trends in Immunology 22:378.
- Groh, V. et al. (1996) Proc. Natl. Acad. Sci. USA 93:12445.
- Bauer, S. et al. (1999) Science 285:727.
- Kawabata, Y. et al. (2000). Human Immunology. 61: 624.
- Bahram, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91:6259.
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