Recombinant Human MICB His-tag Protein, CF

Catalog #: 10431-MB Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10431-MB-050

Recombinant Human MICB His-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human MICB His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human NKG2D/CD314 Fc Chimera (Catalog # 1299-NK) is immobilized at 0.125 μg/mL, 100 μL/well, the concentration of Recombinant Human MICB His-tag (Catalog # 10431-MB) that produces 50% of the optimal binding response is approximately 80-400 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human MICB protein
Ala23-Thr308, with a C-terminal 6-His tag

Accession #

NP_005922.2

N-terminal Sequence
Analysis

Ala23 & Glu24

Predicted Molecular Mass

33 kDa

SDS-PAGE

35-55 kDa, under reducing conditions

Product Datasheets

10431-MB

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10431-MB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human NKG2D/CD314 Fc Chimera (Catalog # 1299-NK) is immobilized at 0.125 µg/mL, 100 µL/mL, the concentration of Recombinant Human MICB His-tag (Catalog # 10431-MB) produces 50% of the optimal binding response is approximately 80-400 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human MICB His-tag Protein (Catalog # 10431-MB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands 35-55 kDa.

Reconstitution Calculator

Background: MICB

Major Histocompatibility Complex Class I Chain-related Gene B (MICB) is a transmembrane glycoprotein that functions as a ligand for human Natural-Killer Group 2 Member D (NKG2D) (1). MICB, along with the highly related MICA, are members of the non-classical MHC class I family. Similar to classical MHC class I proteins, MICB contains three Ig-like domains ( alpha 1, alpha 2, and alpha 3) in the extracellular domain (ECD), a transmembrane segment, and a carboxy-terminal cytoplasmic tail (2). The genes encoding MICA and MICB are found within the MHC on the short arm of human chromosome 6 and occur in most mammalian species but are absent from mouse and rat (2-4). Despite structural similarities, MICA/B only share ~27% amino acid (aa) identity with human MHC class I proteins (3). Both MICA and MICB display a significant degree of polymorphism within the ECD and this enables them to be reconfigured and bind with NKG2D rather than binding with beta 2-microglobulin (4, 5). Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells (6, 7). MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (2, 8-11). Inhibition of MICA/B shedding through targeted antibodies allows anti-tumor immunity to be retained (12).

References

Huang, C. et al. (2018) Sci. Rep. 8:15821.
Bahram, S. et al. (1994) Proc. Natl. Acad. Sci. USA 91:6259.
Cosman, D. et al. (2001) Immunity 14:123.
Groh, V. et al. (2001) Nature Immunol. 2:255.
Stephens, H. (2001) Trends Immunol. 22:378.
Bauer, S. et al. (1999) Science 285:727.
Kawabata, Y. et al. (2000). Human Immunology. 61:624.
Groh, V. et al. (2002) Nature 419:734.
Steinle, A. et al. (2001) Immunogenetics 53:279.
Pende, D. et al. (2002) Cancer Res. 62:6178.
Salih, H. et al. (2003) Blood 102:1389.
Ferrari de Andrade et al. (2018) Science 359:1537.

Long Name

MHC Class I-related Protein B

Entrez Gene IDs

4277 (Human)

Alternate Names

MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I polypeptide-related sequence B; MICB; MIC-B; PERB11.2MHC class I-like molecule PERB11.2-IMX; stress inducible class I homolog