Recombinant Human MMR/CD206 Protein Summary
Product Specifications
Leu19-Lys1383 (Thr399Ala) & (Leu407Phe), with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2534-MR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
2534-MR/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: MMR/CD206
The MMR (macrophage mannose receptor) is also called MR due to its presence on cells other than macrophages, and is designated CD206, Mrc1 (mannose receptor C type 1), or CLEC13D (C‑type lectin domain family 13, member D) (1‑4). CD206 is a 175‑190 kDa endocytic receptor that is expressed on M2 alternatively activated tissue macrophages including tumor‑associated macrophages (TAMs), inflammatory dendritic cells in selected lymphoid organs, and liver, splenic, lymphatic, and dermal microvascular endothelial cells (1, 2, 5‑8). The 1456 amino acid (aa) human CD206 precursor contains a signal sequence (18 aa), an extracellular domain (ECD) containing an N‑terminal cysteine‑rich domain, a fibronectin type II repeat, eight C‑type lectin domains (CTLDs), and several N‑glycosylation sites (1371 aa), a transmembrane segment and a short (46 aa) cytoplasmic domain (2‑4). Metalloproteinases can mediate the shedding of the soluble ECD (2). The human CD206 ECD shares 83%, 84%, 89%, 89% and 90% aa sequence identity with mouse, rat, equine, porcine and canine CD206, respectively. The cysteine‑rich domain recognizes some pituitary hormones such as LH (luteinizing hormone/lutropin) and TSH (thyroid stimulating hormone/thyrotropin), chondroitin sulfates, and sulfated N‑acetylgalactosamines including sulfo‑Lewisa and ‑Lewisx (1, 7, 9). The FNII domain mediates Ca2+‑independent binding of collagens (2, 10). The CTLDs participate in Ca2+‑dependent recognition of branched sugars with terminal mannose, fucose or N‑acetylglucosamine that occur on many pathogenic microorganisms (7, 11). CD206 internalizes ligands in clathrin‑coated vesicles, sorts them to phagosomes or early endosomes, and recycles to the cell surface (1, 6, 7). CD206 also promotes clearance of glycoproteins that promote allergy or ongoing inflammation, such as lysosomal hydrolases and myeloperoxidases (1, 2, 5‑7). It is involved in T cell polarization and production of pro‑ and anti‑inflammatory cytokines (1, 2). It facilitates peptide presentation on MHC II, and cross‑presentation on MHC I which is important for tumor immunogenicity (1, 2, 12). This function may be blocked by engagement of CD206 on TAMs by tumor mucins (8).
- Gazi, U. and L. Martinez-Pomares (2009) Immunobiology 214:554.
- Martinez-Pomares, L. (2012) J. Leukoc. Biol. 92:1177.
- Harris, N. et al. (1992) Blood 80:2363.
- Taylor, M.E. et al. (1990) J. Biol. Chem. 265:12156.
- Chieppa, M. et al. (2003) J. Immunol. 171:4552.
- Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
- Taylor, P.R. et al. (2005) Trends Immunol. 26:104.
- Allavena, P. et al. (2010) Clin. Dev. Immunol. 2010:547179.
- Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
- Martinez-Pomares, L. et al. (2006) Eur. J. Immunol. 36:1074.
- Taylor, M.E. et al. (1992) J. Biol. Chem. 267:1719.
- Singh S.K. et al. (2011) Eur. J. Immunol. 41:916.
Citations for Recombinant Human MMR/CD206 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Transcriptional and functional insights into the host immune response against the emerging fungal pathogen Candida auris
Authors: M Bruno, S Kersten, JM Bain, M Jaeger, D Rosati, MD Kruppa, DW Lowman, PJ Rice, B Graves, Z Ma, YN Jiao, A Chowdhary, G Renieris, FL van de Vee, BJ Kullberg, EJ Giamarello, A Hoischen, NAR Gow, AJP Brown, JF Meis, DL Williams, MG Netea
Nat Microbiol, 2020-08-24;0(0):.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Ozone-derived oxysterols impair lung macrophage phagocytosis via adduction of some phagocytosis receptors
Authors: PF Duffney, HH Kim, NA Porter, I Jaspers
J. Biol. Chem., 2020-07-20;0(0):.
Species: Human
Sample Types: Protein
Applications: ELISA Development -
Mapping of Recognition Sites of Monoclonal Antibodies Responsible for the Inhibition of Pneumolysin Functional Activity
Authors: I Kucinskait, M Simanavici, J Dapkunas, M Pleckaityt, A Zvirbliene
Biomolecules, 2020-07-08;10(7):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA (detection) -
Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma
Authors: J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani
PLoS ONE, 2019-07-25;14(7):e0219480.
Species: Human
Sample Types: Natural Protein
Applications: Bioassay -
N-Oxy lipid-based click chemistry for orthogonal coupling of mannan onto nanoliposomes prepared by microfluidic mixing: Synthesis of lipids, characterisation of mannan-coated nanoliposomes and in vitro stimulation of dendritic cells
Authors: E Bartheldyo, P Turánek Kn, K Zachová, J Mašek, P Kulich, R Effenberg, D Zyka, F Hubatka, J Kotou?ek, H ?elechovsk, R Héžová, A Tome?ková, E Mašková, M Fojtíková, S Macaulay, P Bystrický, L Paulovi?ov, E Paulovi?ov, L Drož, M Ledvina, M Raška, J Turánek
Carbohydr Polym, 2018-11-29;207(0):521-532.
Applications: Binding Assay -
Pneumolysin binds to the mannose receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival
Authors: K Subramania, DR Neill, HA Malak, L Spelmink, S Khandaker, G Dalla Libe, E Dearing, A Kirby, M Yang, A Achour, J Nilvebrant, PÅ Nygren, L Plant, A Kadioglu, B Henriques-
Nat Microbiol, 2018-11-12;0(0):.
Applications: Surface Plasmon Resonance -
A glucose-responsive insulin therapy protects animals against hypoglycemia
Authors: R Yang, M Wu, S Lin, RP Nargund, X Li, T Kelly, L Yan, G Dai, Y Qian, Q Dallas-Yan, PA Fischer, Y Cui, X Shen, P Huo, DD Feng, MD Erion, DE Kelley, J Mu
JCI Insight, 2018-01-11;3(1):.
Applications: Bioassay -
Bee Venom Phospholipase A2, a Novel Foxp3+ Regulatory T Cell Inducer, Protects Dopaminergic Neurons by Modulating Neuroinflammatory Responses in a Mouse Model of Parkinson's Disease.
Authors: Chung E, Lee G, Lee C, Ye M, Chung H, Kim H, Bae S, Hwang D, Bae H
J Immunol, 2015-10-09;195(10):4853-60.
Species: Human
Sample Types: Whole Cells
Applications: Surface Plasmon Resonance -
Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans.
PLoS ONE, 2015-03-18;10(3):e0119244.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
O-mannosylation of the Mycobacterium tuberculosis adhesin Apa is crucial for T cell antigenicity during infection but is expendable for protection.
Authors: Nandakumar, Subhadra, Kannanganat, Sunil, Dobos, Karen M, Lucas, Megan, Spencer, John S, Fang, Sunan, McDonald, Melissa, Pohl, Jan, Birkness, Kristin, Chamcha, Venkates, Ramirez, Melissa, Plikaytis, Bonnie B, Posey, James E, Amara, Rama Rao, Sable, Suraj B
PLoS Pathog, 2013-10-10;9(10):e1003705.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Pichia pastoris-produced mucin-type fusion proteins with multivalent O-glycan substitution as targeting molecules for mannose-specific receptors of the immune system.
Authors: Gustafsson A, Sjoblom M, Strindelius L, Johansson T, Fleckenstein T, Chatzissavidou N, Lindberg L, Angstrom J, Rova U, Holgersson J
Glycobiology, 2011-04-07;21(8):1071-86.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance
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I used this protein as a standard for developing an ELISA in combination with pAb (cat.AF2534) and mAb (cat.MAB25341). This combination gives good standard curve but unfortunately did not detect any MMR in our samples.
