Recombinant Human/Mouse BMPR-II Fc Chimera Protein, CF

Name: Recombinant Human/Mouse BMPR-II Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 811-BR
Rating: 5 (1 reviews)

Catalog #: 811-BR
10 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
811-BR-100

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Citations (10)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human/Mouse BMPR-II Fc Chimera Protein, CF Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Mouse GDF-9 (Catalog # 739-G9) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human/Mouse BMPR‑II Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.1 ‑ 0.5 μg/mL.

Source

Mouse myeloma cell line, NS0-derived BMPR-II protein

Human BMPR-II (Ala26 - Ile151, with an N-terminal Met) Accession # Q13873	IEGRMD	Human IgG₁(Pro100 - Lys330)	6-His tag
N-terminus			C-terminus

Accession #

Q13873

N-terminal Sequence
Analysis

Met & Ser27

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

41.5 kDa (monomer)

SDS-PAGE

65-75 kDa, reducing conditions

Product Datasheets

811-BR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

811-BR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: BMPR-II

Like other members of the TGF-beta superfamily, cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the binding to heteromeric complex of type I and type II serine-threonine kinase receptors. Both receptor types are required for the signl transduction. BMP receptor II (BMPR-II) is one of the five mammalian type II receptors (including TGF-beta R-II, ActR-II, ActR-IIB, BMPR-II and MISR-II) for the TGF-beta superfamily ligands. The type II receptors for TGF-beta and activin bind ligands with high affinity by itself. In contrast, BMPR-II binds BMP-2, BMP-4 and BMP-7 weakly in the absence of type I receptor, and the binding can be facilitated by the presence of the type I receptor. BMPR-II mRNA is widely expressed in fetal and adult tissues. Human and mouse BMPR-II are highly conserved sharing 97% sequence identity.

References

Rosenzweig, B.L. et al. (1995) Proc. Natl. Acad. Sci. USA 92:7632.
Beppu, H. et al. (1997) Biochem. Biophys. Res. Commun. 235:499.
Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.

Long Name

Bone Morphogenetic Protein Receptor II

Entrez Gene IDs

659 (Human); 12168 (Mouse)

Alternate Names

BMP type II receptor; BMP type-2 receptor; BMPR2; BMPR-2; BMPR3; BMPRII; BMPR-II; BMPR-IIprimary pulmonary hypertension 1; Bone morphogenetic protein receptor type II; bone morphogenetic protein receptor type-2; bone morphogenetic protein receptor, type II (serine/threonine kinase); BRK-3; EC 2.7.11; EC 2.7.11.30; FLJ41585; FLJ76945; PPH1BMR2; T-ALK; type II activin receptor-like kinase; type II receptor for bone morphogenetic protein-4

Related Research Areas

Citations for Recombinant Human/Mouse BMPR-II Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Crystal structures of BMPRII extracellular domain in binary and ternary receptor complexes with BMP10
Authors: J Guo, B Liu, M Thorikay, M Yu, X Li, Z Tong, RM Salmon, RJ Read, P Ten Dijke, NW Morrell, W Li
Nature Communications, 2022-05-03;13(1):2395.
Species: Human
Sample Types: Recombinant Proteins
Applications: Surface Plasmon Resonance
The anti-Mï¿½llerian hormone prodomain is displaced from the hormone/prodomain complex upon bivalent binding to the hormone receptor
Authors: RL Cate, N di Clement, C Racine, NP Groome, RB Pepinsky, A Whitty
The Journal of Biological Chemistry, 2021-11-19;0(0):101429.
Species: Human
Sample Types: Recombinant Proteins
Applications: ELISA Capture
BMPR1A is necessary for chondrogenesis and osteogenesis while BMPR1B prevents hypertrophic differentiation
Authors: T Mang, K Kleinschmi, F Ploeger, A Schoeneman, S Lindemann, A Gigout
J. Cell. Sci., 2020-08-21;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance
Facilitated receptor-recognition and enhanced bioactivity of bone morphogenetic protein-2 on magnesium-substituted hydroxyapatite surface
Authors: B Huang, Y Yuan, T Li, S Ding, W Zhang, Y Gu, C Liu
Sci Rep, 2016-04-14;6(0):24323.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Preferential Induction of the T Cell Auxiliary Signaling Molecule B7-H3 on Synovial Monocytes in Rheumatoid Arthritis
Authors: BR Yoon, YH Chung, SJ Yoo, K Kawara, J Kim, IS Yoo, CG Park, SW Kang, WW Lee
J. Biol. Chem, 2015-12-23;291(8):4048-57.
Species: Human
Sample Types:
Applications: Bioassay
Rapid Activation of Bone Morphogenic Protein 9 by Receptor-mediated Displacement of Pro-domains
Authors: Y Kienast, U Jucknischk, S Scheiblich, M Thier, M de Wouters, A Haas, C Lehmann, V Brand, D Bernicke, K Honold, S Lorenz
J. Biol. Chem, 2015-12-16;291(7):3395-410.
Species: Human
Sample Types:
Applications: Bioassay
Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
J. Biol. Chem., 2011-07-07;286(34):30034-46.
Species: Human, Mouse
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance
A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
Authors: Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY
J. Mol. Biol., 2008-07-02;381(4):1025-39.
Species: Human
Sample Types: N/A, Recombinant Protein
Applications: ELISA (Capture), Enzyme Assay, Surface Plasmon Resonance
The BMP-binding protein Crossveinless 2 is a short-range, concentration-dependent, biphasic modulator of BMP signaling in Drosophila.
Authors: Serpe M, Umulis D, Ralston A, Chen J, Olson DJ, Avanesov A, Othmer H, O'Connor MB, Blair SS
Dev. Cell, 2008-06-01;14(6):940-53.
Species: Drosophila
Sample Types: Recombinant Protein
Applications: Binding Assay
Regulation of ADMP and BMP2/4/7 at opposite embryonic poles generates a self-regulating morphogenetic field.
Authors: Reversade B, De Robertis EM
Cell, 2005-12-16;123(6):1147-60.
Species: Human
Sample Types: Recombinant Protein
Applications: Receptor Binding Assay