Recombinant Human Mrc2 His-tag Protein, CF

Catalog #: 10483-EN Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10483-EN-050

Recombinant Human Mrc2 His-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Mrc2 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human Mrc2 His-tag (Catalog # 10483-EN) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Pro-Collagen I alpha 1 (Catalog # 6220-CL) binds with an ED₅₀ of 0.03-0.36 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human Mrc2 protein
Gly31-Ala1414, with a C-terminal 6-His tag

Accession #

AAF14192.1

N-terminal Sequence
Analysis

Gly31

Predicted Molecular Mass

157 kDa

SDS-PAGE

157-203 kDa, under reducing conditions

Product Datasheets

10483-EN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10483-EN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human Mrc2 His-tag (10483-EN) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Pro-Collagen I alpha 1 (6220-CL) binds with an ED₅₀ of 0.03-0.36 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human Mrc2 His-tag (10483-EN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 155-205 kDa.

Reconstitution Calculator

Background: Mrc2

Mrc2 (C-type Mannose Receptor 2), also know as MMR2, Endocytic Receptor 180 and uPARAP, is a 180‑kDa type I transmembrane protein. It is one of the mannose receptor (MR) family members which share a common domain organization and have a broad range of biological functions (1). Mrc2 is an endocytic receptor that is found on migrating cells, including cancer cells, macrophages, fibroblasts and endothelial cells (2). Mature human Mrc2 is composed of 1449 amino acid (aa) that includes a 1384 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 44 aa cytoplasmic domain. The ECD shows one ricin B‑type lectin domain, one fibronectin type II domain and eight C‑type lectin domains. Within the ECD, human Mrc2 shares 91% aa identity with mouse and rat Mrc2. Mrc2 plays an important role in extracellular matrix remodeling through interaction with its ligands, including Man, Fuc, NAcGlc, collagens and urokinase plasminogen activator receptor (uPAR) (1-3). This cell surface molecule has been reported to promote cell invasion through matrix remodeling by internalizing large fragments of collagen and routing it to the lysosome for intracellular degradation and cell chemotaxis (2). It has also been reported to interact with matrix metalloprotease-13 (MMP-13) and collagen V on the cell surface (4).

References

Yuan, C. et al. (2016) Biochem. J. 473:2359.
Durrel, T. et al. (2018) Nat. Commun. 9:5178.
Behrendt, N. et al. (2000) J. Biol. Chem. 275:1993.
Englehom, L.H. et al. (2001) Lab. Invest. 81:1403.

Long Name

Mannose Receptor, C-type 2

Entrez Gene IDs

9902 (Human); 17534 (Mouse); 498011 (Rat)

Alternate Names

CD280 antigen; CD280; CD280C-type mannose receptor 2; CLEC13E; CLEC13EUPAR-associated protein; Endo180; ENDO180C-type lectin domain family 13 member E; Endocytic receptor 180; KIAA0709FLJ35911; Macrophage mannose receptor 2; mannose receptor, C type 2; Mrc2; uPARAP; UPARAPendocytic receptor (macrophage mannose receptor family); urokinase plasminogen activator receptor-associated protein; Urokinase receptor-associated protein; Urokinase-type plasminogen activator receptor-associated protein