Recombinant Human MSP/MST1 (Cys672Ala) Protein Summary
Product Specifications
Gln19-Arg483 (alpha) & Val484-Gly711 (Cys672Ala) (beta)
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4306-MS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
4306-MS/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: MSP/MST1
Macrophage stimulating protein (MSP), also known as HGF-like protein, and scatter factor-2, is a member of the HGF family of growth factors (1). MSP is secreted as an inactive single chain precursor (pro-MSP) that contains a PAN/APPLE-like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity (2). Human MSP shares 79% aa sequence identity with mouse MSP and 44% aa sequence identity with human HGF. Pro-MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF-4 or RAR, respectively (3). Circulating pro-MSP is proteolytically cleaved in response to tissue injury to yield biologically active disulfide linked heterodimers consisting of a 45 - 62 kDa alpha and a 25 - 35 kDa beta chain (4, 5). Pro-MSP can be activated by MT-SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers (6). Heterodimeric MSP, as well as the isolated beta chain, binds to MSP R/Ron with high-affinity, although only heterodimeric MSP can induce receptor dimerization and signaling (7, 8). MSP induces macrophage and keratinocyte proliferation and osteoclast activation (9, 10). It also inhibits LPS- or IFN-induced iNOS and IL-12 expression by macrophages and prevents apoptosis of epithelial cells separated from the ECM (11, 12). The substitution of cysteine 672 (in the beta chain) with alanine significantly increases the bioactivity of recombinant MSP, apparently by limiting incorrect disulfide bond formation between the alpha and beta chains (13).
- Wang, M.-H. et al. (2002) Scand. J. Immunol.56:545.
- Han, S. et al. (1991) Biochemistry 30:9768.
- Muraoka, R.S. et al. (1999) Endocrinology 140:187.
- Wang, M.-H. et al. (1996) J. Clin. Invest. 97:720.
- Nanney, L.B. et al. (1998) J. Invest. Dermatol. 111:573.
- Bhatt, A.S. et al. (2007) Proc. Natl. Acad. Sci. 104:5771.
- Wang, M.-H. et al. (1997) J. Biol. Chem. 272:16999.
- Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
- Wang, M.-H. et al. (1996) Exp. Cell Res. 226:39.
- Kurihara, N. et al. (1998) Exp. Hematol. 26:1080.
- Morrison, A.C. et al. (2004) J. Immunol. 172:1825.
- Liu, Q.P. et al. (1999) J. Immunol. 163:6606.
- Wahl, R.C. et al. (1997) J. Biol. Chem. 272:15053.
Citations for Recombinant Human MSP/MST1 (Cys672Ala) Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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RON (MST1R) and HGFL (MST1) Co-Overexpression Supports Breast Tumorigenesis through Autocrine and Paracrine Cellular Crosstalk
Authors: BG Hunt, A Jones, C Lester, JC Davis, NM Benight, SE Waltz
Cancers, 2022-05-19;14(10):.
Species: Mouse
Sample Types: Organoid
Applications: Bioassay -
Neuroprotective Role of the Ron Receptor Tyrosine Kinase Underlying Central Nervous System Inflammation in Health and Disease
Authors: A Dey, JN Allen, JW Fraser, LM Snyder, Y Tian, L Zhang, RF Paulson, A Patterson, MT Cantorna, PA Hankey-Gib
Front Immunol, 2018-03-19;9(0):513.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The DEK oncogene promotes cellular proliferation through paracrine Wnt signaling in Ron receptor-positive breast cancers.
Authors: Privette Vinnedge L, Benight N, Wagh P, Pease N, Nashu M, Serrano-Lopez J, Adams A, Cancelas J, Waltz S, Wells S
Oncogene, 2014-06-23;34(18):2325-36.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Autoinhibition of the Ron receptor tyrosine kinase by the juxtamembrane domain.
Authors: Wang, Xin, Yennawar, Neela, Hankey, Pamela A
Cell Commun Signal, 2014-04-16;12(0):28.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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