Recombinant Human MuSK His-tag Protein, CF

Catalog #: 11421-MK Datasheet / COA / SDS

(HEK293 expressed)

Catalog #	Availability	Size / Price	Qty
11421-MK-050

Recombinant Human MuSK His-tag Protein Binding Activity.

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human MuSK His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Recombinant Human MuSK His-tag binds to Recombinant Human LRP-4 Protein (Catalog # 5948-LR) / Recombinant Human Agrin Protein (Catalog # 6624-AG) with a ED₅₀ of 0.300-3.00 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human MuSK protein
Leu24-Thr495, with a C-terminal 6-His tag

Accession #

O15146.1

N-terminal Sequence
Analysis

Leu24

Predicted Molecular Mass

53 kDa

SDS-PAGE

58-72 kDa, under reducing conditions.

Product Datasheets

11421-MK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11421-MK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Measured by its binding ability in a functional ELISA. Recombinant Human MuSK His-tag Protein (Catalog # 11421-MK) binds to Recombinant Human LRP-4 Protein (5948-LR / Recombinant Human Agrin Protein (6624-AG) with a ED₅₀ of 0.300-3.00 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human MuSK His-tag Protein (Catalog # 11421-MK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58-72 kDa, under reducing conditions.

Reconstitution Calculator

Background: MuSK

Musk (muscle-specific kinase) is a 53 kDa type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family (1). It contains a 472 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 353 cytoplasmic domain (1). Within the ECD, Human Musk shares 91% and 92% aa sequence identity with mouse Musk and rat Musk, respectively. It is found in the postsynaptic membrane of skeletal muscle motor endplates (2). Human Musk has multiple isoforms. One contains deletions at residues 307‑394 and 454‑461, while a second is a short soluble form that contains residues 120‑209 plus a unique 24 aa C‑terminal tail (3). Musk binds the heparin sulfate proteoglycan Agrin to promote acetylcholine receptor clustering. It has also been found to bind with low-density lipoprotein receptor-related protein 4 (LRP4) (4), Wnt ligands (5), Biglycan (6), and ColQ (7). Recent studies have showed that Musk does not bind Agrin directly, but enhanced the MuSK-LRP4 interaction (7, 8). When Agrin binds to the N-Terminal region of LRP4, this promotes the association of LRP4 and Musk, which then stimulates Musk kinase activity (9, 10).

References