Recombinant Human Nephronectin Protein, CF Summary
Product Specifications
Glu20-Arg565, with Val144Ile, Ile234Val, Asp377Asn substitutions and a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9560-NP
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, EDTA and CHAPS. |
| Reconstitution | Reconstitute at 200 μg/mL in water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Immobilized Recombinant Human Nephronectin supports the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 20-120 ng/mL.
Reconstitution Calculator
Background: Nephronectin
Nephronectin, also named POEM (preosteoblast EGF repeat protein with MAM domain) is a 70-90 kDa extracellular matrix protein that is a ligand for integrin alpha 8 beta 1 (1, 2). The 565 amino acid (aa) human Nephronectin contains a 19 aa signal sequence, a matrilin-type vWA domain, three calcium-binding EGF-like domains, a potential N-linked glycosylation site, a pro/ser/thr-rich mucin-like region, an RGD integrin binding motif, and a MAM domain (1, 3, 4). These features are often present in matrix proteins that, like Nephronectin, mediate cell adhesion and spreading (1, 2, 5). Six human Nephronectin isoforms were identified with alternative splicing. Mature human Nephronectin shows 88% aa identity with both mouse and rat Nephronectin. It is most highly expressed in developing endocrine organs such as parathyroid, thyroid, hypophysis and pineal organ, and around developing bone, teeth, and muscle (1, 2). Pre-osteoblast cells produce Nephronectin, but down-regulate it as they differentiate (1). It is also expressed in the Wolffian duct and ureteric bud basement membranes in the developing kidney, where it is colocalized, and forms an in-vivo complex with integrin alpha 8 beta 1 (2). Since deletion of integrin alpha 8 beta 1 results in renal agenesis, Nephronectin has been proposed to be a critical integrin alpha 8 beta 1 ligand during Nephrogenesis (2, 6).
- Morimura, N. et al. (2001) J. Biol. Chem. 276:42172.
- Brandenberger, R. et al. (2001) J. Cell Biol. 154:447.
- Sato, Y. et al. (2009) J. Biol. Chem. 284:14524.
- Patra, C. et al. (2012) Biomaterials. 33:4327.
- Yamanda, A. and R. Kamijo (2016) J. Dent. & Oral Disord. 2:1004.
- Miner, J. H. (2001) J. Cell Biol. 154:257.
Citation for Recombinant Human Nephronectin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Nephronectin-integrin alpha8 signaling is required for proper migration of periocular neural crest cells during chick corneal development
Authors: J Ma, L Bi, J Spurlin, P Lwigale
Elife, 2022-03-03;11(0):.
Species: Chicken
Sample Types: Whole Cells
Applications: Stimulation
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