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Recombinant Human Netrin-4 Protein

Catalog #: 1254-N4
9 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
1254-N4-025/CF

With Carrier

Catalog # Availability Size / Price Qty
1254-N4-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (9)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Netrin-4 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rrUNC5H2/Fc Chimera at 5 µg/mL (100 µL/well) can bind rhNetrin-4 with a linear range of 6-400 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Netrin-4 protein
Val26-Lys628, with a C-terminal 10-His tag
Accession #
Q9HB63.2
N-terminal Sequence
Analysis
Val26
Predicted Molecular Mass
69 kDa
SDS-PAGE
90 kDa, reducing conditions

Product Datasheets

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1254-N4 (with carrier)

Product Datasheet
COA
SDS

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1254-N4/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1254-N4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1254-N4/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Netrin-4

Netrins/UNC-6 (netr: Sanskrit for “one who guides”) are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins bind to the DCC and UNC5 family of receptors to attract or repel axons. Human Netrin-4 is synthesized as a 628 amino acid (aa) precursor that contains a 19 aa signal sequence, a 443 aa laminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a 166 aa C-terminal domain rich in basic aa residues that serves as a heparin binding site. Unlike human Netrin-1 which is reminiscent of the laminin gamma -chain, human Netrin-4 resembles the laminin beta -chain. Netrin-4 has been reported to exist as both a monomer and a dimer. The dimeric form was reported to be the less active of the two. Human Netrin-4 shares 31%, 29% and 25% aa sequence identity with human Netrin-1, 2 and G2, respectively; it shares 89% aa identity with mouse Netrin-4. Netrin-4 has widespread expression, occurring in nervous tissues such as embryonic floor plate and postnatal neurons such as cerebellar granule cells and hippocampal pyramidal cells. It also is found in non-neural tissues, such as adult Bowman’s capsule and medullary tubular epithelium in kidney, and embryonic pancreatic and intestinal epithelium, plus cells of the ureteric bud. Netrin-4 expression is often associated with basement membrane. Netrin-4 has been shown to initiate axon outgrowth from olfactory bulb explants (1 - 7).

References
  1. McFarlane, S. (2000) Biochem. Cell. Biol. 78:563.
  2. Kennedy, T.E. (2000) Biochem. Cell Biol. 78:569.
  3. Yu, T.W. and C.I. Bargmann (2001) Nature Neurosci.
    4(Suppl):1169.
  4. Stein, E. et al. (2001) Science 291:1976.
  5. Koch, M. et al. (2000) J. Cell Biol. 151:221.
  6. Yin, Y. et al. (2000) Mech. Dev. 96:115.
  7. Livesey, F.J. (1999) Cell. Mol. Life Sci. 56:62.
Entrez Gene IDs
59277 (Human); 57764 (Mouse)
Alternate Names
beta-Netrin; hepar-derived netrin-like protein; netrin 4; Netrin4; Netrin-4; NTN4; PRO3091

Citations for Recombinant Human Netrin-4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Machine Learning Reveals Synovial Fibroblast Genes Associated with Pain Affect Sensory Nerve Growth in Rheumatoid Arthritis
    Authors: Bai, Z;Bartelo, N;Aslam, M;Hale, C;Blachere, NE;Parveen, S;Spolaore, E;DiCarlo, E;Gravallese, E;Smith, MH;Accelerating Medicines Partnership Program: Rheumatoid Arthritis and Systemic Lupus Erythematosus (AMP RA/SLE) Network, ;Frank, MO;Jiang, CS;Zhang, H;Lewis, MJ;Sikandar, S;Pitzalis, C;Malfait, AM;Miller, RE;Zhang, F;Goodman, S;Darnell, R;Wang, F;Orange, DE;
    medRxiv : the preprint server for health sciences
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Netrin-4 expression by human endothelial cells inhibits endothelial inflammation and senescence
    Authors: H Zhang, D Vreeken, DG Leuning, CS Bruikman, A Junaid, W Stam, RG de Bruin, WMPJ Sol, TJ Rabelink, BM van den Be, AJ van Zonnev, JM van Gils
    The international journal of biochemistry & cell biology, 2021-02-23;134(0):105960.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  3. Isolation and characterization of myogenic precursor cells from human cremaster muscle
    Authors: N Naldaiz-Ga, M Goicoechea, IM Aragón, V Pérez-Lópe, S Fuertes-Al, B Herrera-Im, A López de M, R de Luna-Di, PM Baptista, MA Fernández, MF Lara, A Izeta
    Sci Rep, 2019-03-05;9(1):3454.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. EGF/EGFR upregulates and cooperates with Netrin-4 to protect glioblastoma cells from DNA damage-induced senescence
    Authors: L Li, Y Huang, Y Gao, T Shi, Y Xu, H Li, M Hyytiäinen, J Keski-Oja, Q Jiang, Y Hu, Z Du
    BMC Cancer, 2018-12-04;18(1):1215.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. The Netrin-4/ Neogenin-1 axis promotes neuroblastoma cell survival and migration
    Authors: AA Villanueva, P Falcón, N Espinoza, LS R, LA Milla, E Hernandez-, VA Torres, P Sanchez-Go, V Palma
    Oncotarget, 2017-02-07;8(6):9767-9782.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Netrin-1 promotes medulloblastoma cell invasiveness and angiogenesis, and demonstrates elevated expression in tumor tissue and urine of patients with pediatric medulloblastoma.
    Authors: Akino T, Han X, Nakayama H, McNeish B, Zurakowski D, Mammoto A, Klagsbrun M, Smith E
    Cancer Res, 2014-05-08;74(14):3716-26.
    Applications: Western Blot
  7. NETRIN-4 protects glioblastoma cells FROM temozolomide induced senescence.
    Authors: Li L, Hu Y, Ylivinkka I, Li H, Chen P, Keski-Oja J, Hyytiainen M
    PLoS ONE, 2013-11-12;8(11):e80363.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme.
    Authors: Sneddon, Julie B, Borowiak, Malgorza, Melton, Douglas
    Nature, 2012-10-07;491(7426):765-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  9. Netrin-4 inhibits angiogenesis via binding to neogenin and recruitment of Unc5B.
    Authors: Lejmi E, Leconte L, Pedron-Mazoyer S, Ropert S, Raoul W, Lavalette S, Bouras I, Feron JG, Maitre-Boube M, Assayag F, Feumi C, Alemany M, Jie TX, Merkulova T, Poupon MF, Ruchoux MM, Tobelem G, Sennlaub F, Plouet J
    Proc. Natl. Acad. Sci. U.S.A., 2008-08-21;105(34):12491-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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