Recombinant Human Netrin-G2 His-tag Protein, CF

Catalog #: 10675-NG Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10675-NG-050

Recombinant Human Netrin-G2 His-tag Protein Binding Activity.

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Netrin-G2 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Netrin‑G2 His-Tag protein is coated at 1 µg/mL, Recombinant Human LRRC4 Fc Chimera (Catalog # 9375-LR) binds with an ED₅₀ of 1.5-9 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human Netrin-G2 protein
Asp18-Pro506, with a C-terminal 6-His tag

Accession #

Q96CW9.2

N-terminal Sequence
Analysis

Asp18

Predicted Molecular Mass

56 kDa

SDS-PAGE

70-78 kDa, under reducing conditions

Product Datasheets

10675-NG

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10675-NG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human Netrin‑G2 His-Tag (Catalog # 10675-NG) is coated at 1 µg/mL, Recombinant Human LRRC4 Fc Chimera (9375-LR) binds with an ED₅₀ of 1.5-9 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human Netrin-G2 His-tag (Catalog # 10675-NG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-78 kDa.

Reconstitution Calculator

Background: Netrin-G2

NTNG-2 (Netrin-G2) is a member of a GPI-linked Netrin subfamily that is distantly related to classical Netrins. Mature human Netrin-G2 is approximately 490 amino acid (aa) in length. It contains an N-terminal laminin-related region, several epidermal-growth-factor-like domains and a heparin-binding C-terminus that ends in a GPI-linkage (1). The laminin-related region shows a globular type VI domain followed by three variable-length EGF-like domains. Mature human Netrin-G2 shares 96% aa identity with mouse and rat Netrin-G2. NTNG-2 is predominantly expressed in specific neuronal subsets of the developing and mature central nervous system (2). The protein interacts with the extracellular region of their specific Netrin-G ligand receptors LRRC4 (3). The netrin family proteins provide axonal guidance cues during central nervous system development (4).

References

Seiradake E. et al. (2011) The EMBO J. 30:4479.
Pirone A. et al. (2012) PLOS 7(9):e44745.
Woo, J. et al. Mol. Cell Neurosci. (2009) 42:1.
Zhang, Q. et al. (2005) FEBS Lett. 579:3674.

Long Name

Netrin-G2

Entrez Gene IDs

84628 (Human)

Alternate Names

bA479K20.1; KIAA0625; KIAA1857bA479K20.1 (novel protein); laminet 2; laminet-2; LHLL9381; Lmnt2; MGC21884; netrin G1; netrin G2; Netrin-G2; NTNG1; NTNG2