Recombinant Human Neurexophilin-1/NXPH1 Protein, CF

Catalog #: 4957-NX Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
4957-NX-050

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Recombinant Human Neurexophilin-1/NXPH1 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a competitive binding assay. When rrNeurexin-1 alpha (Catalog # 4485-NX) is immobilized at 1 µg/mL (100 µL/well), rhNeurexophilin-1 inhibits 50% binding of biotinylated rrNeurexophilin-1 (250 ng/mL) at the concentration range of 1‑3 µg/mL.

Source

Mouse myeloma cell line, NS0-derived human Neurexophilin-1 protein
Ala22-Gly271, with a C-terminal 6-His tag

Accession #

P58417

N-terminal Sequence
Analysis

Ala22

Predicted Molecular Mass

29.4 kDa

SDS-PAGE

40-60 kDa, reducing conditions

Product Datasheets

4957-NX

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4957-NX

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Neurexophilin-1

Neurexophilin-1 (NXPH1) is one of at least four vertebrate neuropeptide-like secreted glycoproteins in the neurexophilin family (1, 2). The 271 amino acid (aa) NXPH1 sequence contains a 21 aa signal peptide, an 86 aa propeptide that is cleaved at a basic motif, and a 164 aa mature protein that contains three potential N-glycosylation sites in the N-terminal portion and six conserved cysteines in the C-terminal portion (1). Mature human NXPH1 shares 99% aa identity with mouse, rat, equine, bovine and canine NXPH1. NXPH1 is expressed selectively in subpopulations of neurons within the cerebral cortex, cerebellum and olfactory bulb that are thought to be inhibitory interneurons (2, 3). In humans, NXPH-2 and -3 are most similar to NXPH1, sharing 84% and 64% aa identity within the mature region, respectively. In rodents, which do not express NXPH-2, expression of NXPH1 and NXPH-3 does not appear to coincide. However, both serve as tightly bound extracellular ligands for alpha -neurexins, synaptic transmembrane molecules that are essential for calcium-triggered neurotransmitter release (1, 4, 5). By contrast, NXPH-4 does not bind alpha -neurexins (1, 4). Genetic deletion of NXPH1 and/or NXPH-3 produces no anatomical effect, although mice lacking NXPH-3 show defects in motor coordination (4, 6).

References