Recombinant Human Neuroligin 1v2/NLGN1v2 Protein, CF
Recombinant Human Neuroligin 1v2/NLGN1v2 Protein, CF Summary
Product Specifications
Met1-Ser677, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7066-NL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 300 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Neuroligin 1/NLGN1
Neuroligin 1 (NLGN1 or NL1) is one of several type I transmembrane Neuroligin proteins that play an important role in synaptic development (1). Mature human Neuroligin 1 is an N- and O-glycosylated 120 kDa molecule that consists of a 649 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 125 aa cytoplasmic tail (2). In rat, insertion of 20 aa at splice site A and/or 9 aa at splice site B within the ECD results in NLGN1A, NLGN1B, or NLGN1AB isoforms while the NLGN1(-) isoform has neither insertion (3). This recombinant protein lacks the splice site A insert but contains the splice site B insert and corresponds to the human equivalent of rat NLGN1B. Within the ECD, human NLGN1A shares 99% aa sequence identity with comparable regions of mouse and rat Neuroligin 1, respectively. Neuroligin 1 expression is restricted to postsynaptic nerve terminal membranes where it associates into dimers and tetramers (5 ‑ 7). NLGN1B and NLGN1AB are enriched at excitatory (glutamatergic) contacts, whereas NLGN1A is enriched at inhibitory (GABAergic) contacts and NLGN1(-) is expressed at both (3). The interaction of Neuroligin 1 with presynaptic membrane Neurexins promotes the maturation of presynaptic as well as postsynaptic structures (3, 4, 8 ‑ 11). Neuroligin 1 isoforms are selective in which Neurexin isoforms they bind: NLGN1B and NLGN1AB preferentially interact with Neurexin 1 beta lacking the splice site 4 insert, while NLGN1A and NLGN1(-) bind Neurexin 1 alpha and Neurexin 1 beta irrespective of the SS4 insert (12). Neurexin 1 beta (-SS4) additionally binds to LRRTM2 to trigger a second mechanism that promotes excitatory synapse development (13, 14). Neuroligin 1 mediated synaptogenesis is also enhanced by its direct binding to Thrombospondin 1 (15).
- Sudhof, T.C. (2008) Nature 455:903.
- Ichtchenko, K. et al. (1995) Cell 81:435.
- Chih, B. et al. (2006) Neuron 51:171.
- Dean, C. et al. (2003) Nat. Neurosci. 7:708.
- Comoletti, D. et al. (2006) Biochemistry 45:12816.
- Berninghausen, O. et al. (2007) J. Neurochem. 103:1855.
- Song, J.-Y. et al. (1999) Proc. Natl. Acad. Sci. 96:1100.
- Wittenmayer, N. et al. (2009) Proc. Natl. Acad. Sci. 106:13564.
- Jung, S.-Y. et al. (2010) Proc. Natl. Acad. Sci. 107:4710.
- Chubykin, A.A. et al. (2007) Neuron 54:919.
- Chih, B. et al. (2005) Science 307:1324.
- Boucard, A.A. et al. (2005) Neuron 48:229.
- Siddiqui, T.J. et al. (2010) J. Neurosci. 30:7495.
- Ko, J. et al. (2009) Neuron 64:791.
- Xu, J. et al. (2010) Nat. Neurosci. 13:22.
Citations for Recombinant Human Neuroligin 1v2/NLGN1v2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Identification of ligand-receptor pairs that drive human astrocyte development
Authors: Voss, AJ;Lanjewar, SN;Sampson, MM;King, A;Hill, EJ;Sing, A;Sojka, C;Bhatia, TN;Spangle, JM;Sloan, SA;
Nature neuroscience
Species: Human
Sample Types: Organoid
Applications: Bioassay -
Neuroligin-1 in brain and CSF of neurodegenerative disorders: investigation for synaptic biomarkers
Authors: E Camporesi, T Lashley, J Gobom, J Lantero-Ro, O Hansson, H Zetterberg, K Blennow, B Becker
Acta neuropathologica communications, 2021-02-01;9(1):19.
Species: Human
Sample Types: Tissue Homogenates
Applications: Reference Standard -
Targeting neuronal activity-regulated neuroligin-3 dependency in high-grade glioma
Authors: HS Venkatesh, LT Tam, PJ Woo, J Lennon, S Nagaraja, SM Gillespie, J Ni, DY Duveau, PJ Morris, JJ Zhao, CJ Thomas, M Monje
Nature, 2017-09-20;549(7673):533-537.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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