Recombinant Human Neuroligin 3/NLGN3 Protein, CF
Recombinant Human Neuroligin 3/NLGN3 Protein, CF Summary
Product Specifications
Ala39-Ser709 (Ser92Tyr), with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9069-NL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Human Neuroligin 3/NLGN3 (Catalog # 9069-NL) is immobilized at 2 µg/mL, Biotinylated Recombinant Human Neurexin 1 beta Fc Chimera binds with a typical ED50 of 150-900 ng/mL.
Reconstitution Calculator
Background: Neuroligin 3/NLGN3
Neuroligin 3 (NLGN3) is one of several type I transmembrane Neuroligin proteins that play an important role in synaptic development (1, 2). Mature human Neuroligin 3 is an approximately 120 kDa molecule that consists of a 672 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 118 aa cytoplasmic tail (3). Alternative splicing generates additional isoforms with 20 aa or 40 aa deletions in the ECD (3-5). Within the ECD, human Neuroligin 3 shares 99% aa sequence identity with comparable regions of mouse and rat Neuroligin 3. It is expressed during development on astrocytes and Schwann cells (6) as well as on neurons where it localizes to synaptic membranes and post-synaptic densities of both glutamatergic and GABAergic synapses (7). It associates in cis with Neuroligin 1 and Neuroligin 2 and trans-synaptically with Neurexin-1 beta, -2 beta, and -3 beta that lack the splice site 4 insertion (3, 7). A soluble form of the Neuroligin 3 ECD can be released by neurons and promotes glioma cell proliferation (8). Mutations in Neuroligin 3 enhance neuronal dendritic branching, alter both excitatory and inhibitory synaptic activity (9-12), and are associated with the development of autism spectrum disorder (13, 14).
- Bemben, M.A. et al. (2015) Trends Neurosci. 38:496.
- Mackowiak, M. et al. (2014) Pharmacol. Rep. 66:830.
- Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
- Philibert, R.A. et al. (2000) Gene 246:303.
- Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
- Gilbert, M. et al. (2001) Glia 34:151.
- Budreck, E.C. and P. Scheiffele (2007) Eur. J. Neurosci. 26:1738.
- Venkatesh, H.S. et al. (2015) Cell 161:803.
- Etherton, M. et al. (2011) Proc. Natl. Acad. Sci. USA 108:13764.
- Foldy, C. et al. (2013) Neuron 78:498.
- Tabuchi, K. et al. (2007) Science 318:71.
- Gutierrez, R.C. et al. (2009) Neuroscience 162:208.
- Jamain, S. et al. (2003) Nat. Genet. 34:27.
- Rothwell, P.E. et al. (2014) Cell 158:198.
Citations for Recombinant Human Neuroligin 3/NLGN3 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Neuroligin-1 in brain and CSF of neurodegenerative disorders: investigation for synaptic biomarkers
Authors: E Camporesi, T Lashley, J Gobom, J Lantero-Ro, O Hansson, H Zetterberg, K Blennow, B Becker
Acta neuropathologica communications, 2021-02-01;9(1):19.
Species: Human
Sample Types: Tissue Homogenates
Applications: Reference Standard -
Rapid and Efficient Invasion Assay of Glioblastoma in Human Brain Organoids
Authors: G Goranci-Bu, A Mariappan, E Gabriel, A Ramani, L Ricci-Viti, M Buccarelli, QG D'Alessand, R Pallini, J Gopalakris
Cell Rep, 2020-06-09;31(10):107738.
Species: Human
Sample Types: Organoids
Applications: Bioassay -
Interaction of A? Oligomers with Neurexin 2? and Neuroligin 1 Mediates Synapse Damage and Memory Loss in Mice
Authors: J Brito-More, MV Lourenco, MM Oliveira, JF Vital, FC Ribeiro, JH Ledo, LP Diniz, MH Magdesian, HM Melo, F Barros-Ara, JM de Souza, SV Alves-Leon, FC Gomes, JR Clarke, CP Figueiredo, FG De Felice, ST Ferreira
J. Biol. Chem, 2017-03-10;0(0):.
Species: Mouse
Sample Types: Protein
Applications: Bioassay
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