Recombinant Human NgR3/NgRH2 Protein, CF

Catalog #: 8439-NG Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8439-NG-025

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Recombinant Human NgR3/NgRH2 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human APP695 is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Human NgR3/NgRH2 that produces 50% optimal binding response is 0.05-0.25 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human NgR3/NgRH2 protein
Cys25-Ala419, with a C-terminal 6-His tag

Accession #

Q86UN2

N-terminal Sequence
Analysis

Cys25

Predicted Molecular Mass

45 kDa

SDS-PAGE

56-65 kDa, reducing conditions

Product Datasheets

8439-NG

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8439-NG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: NgR3/NgRH2

NgR3/NgRH2 (also known as reticulon-4 receptor-Like 1), along with the related NgR1 and NgR2, is a member of the Nogo66 receptor (NgR) family. NgR proteins are expressed primarily by neurons in the central and peripheral mammalian nervous system. NgR proteins play an essential role in mediating axon growth inhibition induced by the structurally distinct proteins Nogo, myelin associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (OMgp) (1, 2).These proteins belong to the family of myelin-associated inhibitors (MAIs). NgR proteins are composed of 8 leucine-rich repeats (LRRs) flanked by N-terminal and C-terminal leucine-rich capping domains and are attached at the cell surface by a C-terminal glycosylphosphatidylinositol (GPI) anchor (3). Because NgR proteins have no transmembrane domain, they must be associated with membrane proteins in order to transduce cell signaling (2). Both NGF R/TNFRSF16 (p75) and TROY/TNFRSF19 have been identified as NgR1-associated components (4, 5). Human NgR3 shares 88% and 86% amino acid sequence identity with mouse and rat NgR3 respectively. NgR1 and NgR3 serve as receptors for chondroitin sulfate proteoglycans (CSPGs) (6). NgR3 may function as a NgR1 co-receptor for Nogo66 (7). CSPGs and MAIs are known to stabilize neuronal structure and influence the growth and guidance of developing neurons (8). Therefore, NgR proteins are part of a multicomponent receptor system implicated in pathways that limit neuronal growth and increase the structural stability of synapses.

References

GrandPre, T. et al. (2002) Nature 417:547.
Wang, K.C. et al. (2002) Nature 420:74.
Fournier, A.E. et al. (2001) Nature 409:341.
Shao, Z. et al. (2005) Neuron 45:353.
Park, J. B. et al. (2005) Neuron 45:345.
Dickendesher, T.L. et al. (2012) Nat. Neurosci. 15:703.
Zang, G. et al. (2011) J. Genet. Genomics 38:515.
Kantor, D.B. et al. (2004) Neuron 44:961.

Long Name

Nogo-66 Receptor-related Protein 3/Nogo-66 Receptor Homolog 2/Reticulon-4 Receptor-like 1

Entrez Gene IDs

146760 (Human); 237847 (Mouse)

Alternate Names

DKFZp547J144; NgR3; NgRH2; NGRL2; nogo receptor-like 2; Nogo-66 receptor homolog 2; Nogo-66 receptor-related protein 3; reticulon 4 receptor-like 1; reticulon-4 receptor-like 1; RTN4RL1