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Recombinant Human Nodal Protein

Catalog #: 3218-ND
22 Citations 2 Reviews Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
3218-ND-025/CF

With Carrier

Catalog # Availability Size / Price Qty
3218-ND-025
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Product Details
Citations (22)
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Reviews (2)
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Recombinant Human Nodal Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Yeo, C. et al. (2001) Mol. Cell. 7:949. Approximately 100 ng/mL of Recombinant Human Nodal can effectively induce Smad2 phosphorylation.
Source
E. coli-derived human Nodal protein
His238-Leu347, with an N-terminal Met
Accession #
Q96S42
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
12.9 kDa (monomer)

Product Datasheets

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3218-ND (with carrier)

Product Datasheet
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3218-ND/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3218-ND

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3218-ND/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nodal

Nodal is a 13 kDa member of the TGF-beta superfamily of molecules (1-3). Its name derives from the fact that it is expressed in the primitive node of the developing embryo. In human, it is synthesized as a 347 amino acid (aa) preproprecursor that contains a 26 aa signal sequence, a 211 aa prodomain, and a 110 aa mature region (4, 5). Consistent with its TGF-beta superfamily membership, it exists as a disulfide-linked homodimer and would be expected to demonstrate a cysteine-knot motif (1, 5, 6). Mature human Nodal is 99%, 98%, 96% and 98% aa identical to mature canine, rat, bovine and mouse Nodal, respectively. Nodal signals through two receptor complexes, both of which contain members of the TGF-beta family of Ser/Thr kinase receptors. The first receptor complex is composed of ActRIB/ALK4 and ActRIIB. Nodal interaction with the ligand-binding subunit ALK4 requires the participation of fucosylated Cripto, a member of the EGF-CFC gene family. The second complex is composed of ALK7 and ActRIIB, and does not require Cripto interaction (7-9). Nodal signaling is regulated by a number of inhibitors. Nodal is reported to form nonsignaling disulfide-linked heterodimers with BMP-3, -4 and -7 (2, 6). There are also at least three secreted factors that block Nodal activation of its receptor. The first is Cerberus, a member of the DAN family of proteins. The last two are TGF-beta family members named Lefty-A and B (2, 11). The exact mechanisms employed are unclear, but Leftys are apparently induced by nodal and can work at a considerable distance from their origin (2). Nodal is known to induce both mesoderm and endoderm, participate in anterior-posterior positioning, and through ALK7, induce apoptosis in susceptible cell populations (2, 8, 12).

References
  1. Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
  2. Schier, A.F. (2003) Annu. Rev. Cell Dev. Biol. 19:589.
  3. Whitman, M. and M. Mercola (2001) Sci. STKE. Jan 9;(64):RE1.
  4. Gebbia, M. et al. (1997) Nat. Genet. 17:305.
  5. Hart, A.H. et al. (2005) Biochem. Biophys. Res. Commun. 333:1361.
  6. Yeo, C-Y. and M. Whitman (2001) Mol. Cell 7:949.
  7. Yan, Y-T. et al. (2002) Mol. Cell. Biol. 22:4439.
  8. Munir, S. et al. (2004) J. Biol. Chem. 279:31277.
  9. Shen, M.M. and A.F. Schier (2000) Trends Genet. 16:303.
  10. Lah, M. et al. (1999) Genomics 55:364.
  11. Kosaki, K. et al. (1999) Am. J. Hum. Genet. 64:712.
  12. Zhou, X. et al. (1993) Nature 361:543.
Entrez Gene IDs
4838 (Human); 18119 (Mouse)
Alternate Names
BMP-16; MGC138230; nodal homolog (mouse); nodal homolog; Nodal; nodal, mouse, homolog

Citations for Recombinant Human Nodal Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Disruption of TGF-? signaling pathway is required to mediate effective killing of hepatocellular carcinoma by human iPSC-derived NK cells
    Authors: Thangaraj, JL;Coffey, M;Lopez, E;Kaufman, DS;
    Cell stem cell
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Biomarker screen for efficacy of oncolytic virotherapy in patient-derived pancreatic cancer cultures
    Authors: Schäfer, TE;Knol, LI;Haas, FV;Hartley, A;Pernickel, SCS;Jády, A;Finkbeiner, MSC;Achberger, J;Arelaki, S;Modic, Ž;Schröer, K;Zhang, W;Schmidt, B;Schuster, P;Haferkamp, S;Doerner, J;Gebauer, F;Ackermann, M;Kvasnicka, HM;Kulkarni, A;Bots, STF;Kemp, V;Hawinkels, LJAC;Poetsch, AR;Hoeben, RC;Ehrhardt, A;Marchini, A;Ungerechts, G;Ball, CR;Engeland, CE;
    EBioMedicine
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Monitoring phosphorylation and acetylation of CRISPR-mediated HiBiT-tagged endogenous proteins
    Authors: Alves, J;Schwinn, M;Machleidt, T;Goueli, SA;Cali, JJ;Zegzouti, H;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Establishment of Skeletal Myogenic Progenitors from Non-Human Primate Induced Pluripotent Stem Cells
    Authors: Baik, J;Ortiz-Cordero, C;Magli, A;Azzag, K;Crist, SB;Yamashita, A;Kiley, J;Selvaraj, S;Mondragon-Gonzalez, R;Perrin, E;Maufort, JP;Janecek, JL;Lee, RM;Stone, LH;Rangarajan, P;Ramachandran, S;Graham, ML;Perlingeiro, RCR;
    Cells
    Species: Macaca fascicularis (cynomolgus monkey)
    Sample Types: Whole Cells
    Applications: Bioassay
  5. A vector-encoded bispecific killer engager to harness virus-activated NK cells as anti-tumor effectors
    Authors: A Floerching, JE Klein, MSC Finkbeiner, TE Schäfer, G Fuchs, J Doerner, H Zirngibl, M Ackermann, HM Kvasnicka, KA Chester, D Jäger, CR Ball, G Ungerechts, CE Engeland
    Cell Death & Disease, 2023-02-10;14(2):104.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. TGFbeta superfamily signaling regulates the state of human stem cell pluripotency and capacity to create well-structured telencephalic organoids.
    Authors: Watanabe M, Buth J, Haney J, Vishlaghi N, Turcios F, Elahi L, Gu W, Pearson C, Kurdian A, Baliaouri N, Collier A, Miranda O, Dunn N, Chen D, Sabri S, Torre-Ubieta L, Clark A, Plath K, Christofk H, Kornblum H, Gandal M, Novitch B
    Stem Cell Reports, 2022-09-29;17(10):2220-2238.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Recurrent NOMO1 Gene Deletion Is a Potential Clinical Marker in Early-Onset Colorectal Cancer and Is Involved in the Regulation of Cell Migration
    Authors: J Pérez-Garc, A Martel-Mar, P García-Val, LA Corchete, JL García, N Gestoso-Uz, R Vidal-Toci, Ó Blanco, L Méndez, M Sánchez-Ma, M Fuentes, AB Herrero, AN Holowatyj, J Perea, R González-S
    Cancers, 2022-08-20;14(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  8. Influence of Nodal signalling on pluripotency factor expression, tumour cell proliferation and cisplatin-sensitivity in testicular germ cell tumours
    Authors: K Harpelunde, JE Nielsen, B Grønkær To, UN Joensen, LJ Rasmussen, M Blomberg J, RT Mitchell, A Juul, E Rajpert-De, A Jørgensen
    BMC Cancer, 2020-04-23;20(1):349.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. In Vitro CRISPR/Cas9-Directed Gene Editing to Model LRRK2 G2019S Parkinson's Disease in Common Marmosets
    Authors: SC Vermilyea, A Babinski, N Tran, S To, S Guthrie, JH Kluss, JK Schmidt, GJ Wiepz, MG Meyer, ME Murphy, MR Cookson, ME Emborg, TG Golos
    Sci Rep, 2020-02-26;10(1):3447.
    Species: Primate - Callitrix jacchus (Common Marmoset)
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Intrinsic cellular chirality regulates left-right symmetry breaking during cardiac looping
    Authors: P Ray, AS Chin, KE Worley, J Fan, G Kaur, M Wu, LQ Wan
    Proc. Natl. Acad. Sci. U.S.A., 2018-11-20;0(0):.
    Species: Chicken
    Sample Types: Embryo
    Applications: Bioassay
  11. Nodal Signaling Regulates Germ Cell Development and Establishment of Seminiferous Cords in the Human Fetal Testis
    Authors: A Jørgensen, J Macdonald, JE Nielsen, KR Kilcoyne, S Perlman, L Lundvall, L Langhoff T, K Juul Hare, H Frederikse, AM Andersson, NE Skakkebæk, A Juul, RM Sharpe, E Rajpert-De, RT Mitchell
    Cell Rep, 2018-11-13;25(7):1924-1937.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. ALDH enzyme activity is regulated by Nodal and histamine in the A549 cell line
    Authors: Y Jiang, H Li, Y Wang, T Tian, Y He, Y Jin, C Han, X Jin, F Zhang, E Morii
    Oncol Lett, 2017-09-25;14(6):6955-6961.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Succession of transiently active tumor-initiating cell clones in human pancreatic cancer xenografts
    Authors: CR Ball, F Oppel, KR Ehrenberg, TD Dubash, SM Dieter, CM Hoffmann, U Abel, F Herbst, M Koch, J Werner, F Bergmann, N Ishaque, M Schmidt, C von Kalle, C Scholl, S Fröhling, B Brors, W Weichert, J Weitz, H Glimm
    EMBO Mol Med, 2017-07-01;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Bioassay
  14. A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO
    Authors: Taroh Kinoshita
    J. Cell Biol., 2016-11-23;215(5):705-718.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Nodal.Gdf1 heterodimers with bound prodomains enable serum-independent nodal signaling and endoderm differentiation.
    Authors: Fuerer C, Nostro M, Constam D
    J Biol Chem, 2014-05-05;289(25):17854-71.
    Species: Human
    Sample Types: Antibody, Whole Cells
    Applications: Bioassay, Western Blot
  16. Designer nodal/BMP2 chimeras mimic nodal signaling, promote chondrogenesis, and reveal a BMP2-like structure.
    Authors: Esquivies L, Blackler A, Peran M, Rodriguez-Esteban C, Izpisua Belmonte J, Booker E, Gray P, Ahn C, Kwiatkowski W, Choe S
    J Biol Chem, 2013-12-05;289(3):1788-97.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Nodal promotes invasive phenotypes via a mitogen-activated protein kinase-dependent pathway.
    Authors: Quail, D F, Zhang, G, Findlay, S D, Hess, D A, Postovit, L-M
    Oncogene, 2013-01-21;33(4):461-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011-07-07;286(34):30034-46.
    Species: Human, Mouse
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  20. A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
    Authors: Herrera B, Inman GJ
    BMC Cell Biol., 2009-03-19;10(0):20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Requirement of glycosylphosphatidylinositol anchor of Cripto-1 for trans activity as a Nodal co-receptor.
    Authors: Watanabe K, Hamada S, Bianco C, Mancino M, Nagaoka T, Gonzales M, Bailly V, Strizzi L, Salomon DS
    J. Biol. Chem., 2007-10-09;282(49):35772-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed.
    Authors: McLean AB, D&amp;apos;Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S
    Stem Cells, 2007-01-01;25(1):29-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What is the half-life of this BMP protein?

    • R&D Systems has not determined the half-life of this recombinant human BMP protein. Assay conditions for each protein will need to be optimized by each researcher.

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Reviews for Recombinant Human Nodal Protein

Average Rating: 4.5 (Based on 2 Reviews)

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Recombinant Human Nodal Protein
By Anonymous on 10/09/2020
Application: CellProlif
Recombinant Human Nodal Protein
By Anonymous on 12/06/2018
Application: Stem/Immune cell maintenance or differentiation

Picture shows my marmoset embryonic stem cells grown with media that is supplemented with this human nodal protein. Cells are healthy, and have a homogenous morphology without spontaneous differentiation

Stem/Immune cell maintenance or differentiation Recombinant Human Nodal Protein 3218-ND