Recombinant Human NRG1 isoform 9/GGF-2 Protein, CF

Catalog #: 11505-NR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11505-NR-050

Recombinant Human NRG1 isoform 9/GGF-2 Protein Bioactivity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human NRG1 isoform 9/GGF-2 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED₅₀ for this effect is 0.750-7.50 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human Neuregulin-1/NRG1 protein
(isoform 9/GGF-2) Gly51-Glu422
Accession # Q02297-9

Accession #

Q02297-9

N-terminal Sequence
Analysis

Gly51

Predicted Molecular Mass

40 kDa

SDS-PAGE

70-80 kDa, under reducing conditions.

Product Datasheets

11505-NR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11505-NR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED₅₀ for this effect is 0.750-7.50 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human NRG1 isoform 9/GGF-2 Protein (Catalog # 11505-NR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70‑80 kDa, under reducing conditions.

Reconstitution Calculator

Background: Neuregulin-1/NRG1

Glial growth factor2, GGF-2, also known as cimaglermin alfa or NRG1 beta 3 (1), is a full-length splice variant of the Neuregulin-1 (NRG1) family gene. NRG1 is one of the four members of the neuregulin family of genes (NRG1-4) encoding signaling proteins that facilitate cell–cell interactions in many tissues, including the nervous system and the heart (2). All family members share an EGF-like domain that interacts with the ErbB family of tyrosine kinase receptors. Through their displayed interaction with ERBB receptors, NRG1 isoforms induce the growth and differentiation of epithelial, neuronal, glial, and other types of cells. The ErbB2 and ErbB4 receptors cooperate in transmission of neuregulin-1 signals in the heart, whereas ErbB2 and ErbB3 cooperate in neural crest cells.Neuregulins are emerging as potential treatments for heart failure. GGF-2 was chosen as a development candidate with potentially long-lasting pharmacodynamic activity (4).

References

Dimayuga, F.O. et al. (2003) J Neuroimmunol 136:67.
Falls D.L. (2003). Exp. Cell Res. 284:14.
Marchionni M.A. et al. (1999) Adv Exp Med Biol. 468:283.
Caggiano A.O. et al. (2017) Eur J Pharmacol.796:76.

Entrez Gene IDs

3084 (Human); 211323 (Mouse); 112400 (Rat)

Alternate Names

ARIA; GGF2; GGFglial growth factor; Heregulin-1; HGL; HGLneu differentiation factor; HRG; HRG1; HRG1-alpha; HRG1-beta 1; HRGAneuregulin 1 type IV beta 1a; MST131; MSTP131; NDFheregulin, alpha (45kD, ERBB2 p185-activator); neuregulin 1 type IV beta 3; neuregulin 1; Neuregulin1; Neuregulin-1; NRG1; pro-neuregulin-1, membrane-bound isoform; pro-NRG1; sensory and motor neuron derived factor; SMDF