Recombinant Human OX40 Ligand/TNFSF4 His-tag Protein, CF

Catalog #: 11241-OX Datasheet / COA / SDS

HEK293-expressed

Catalog #	Availability	Size / Price	Qty
11241-OX-050

Recombinant Human OX40 Ligand/TNFSF4 His-tag Protein Bioactivity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human OX40 Ligand/TNFSF4 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human OX40/TNFRSF4. The ED₅₀ for this effect is 1.00-15.0 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human OX40 Ligand/TNFSF4 protein
Gln51-Leu183, with a N-terminal Met-10-His tag

Accession #

P23510.1

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

18 kDa

SDS-PAGE

17-34 kDa, under non-reducing conditions.

Product Datasheets

11241-OX

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11241-OX

Formulation	Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Bioactivity

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Recombinant Human OX40 Ligand/TNFSF4 His-tag Protein (Catalog # 11241-OX) induces IL-8 secretion in the HT1080 human fibrosarcoma cell line. The ED₅₀ for this effect is 1.00-15.0 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human OX40 Ligand/TNFSF4 His-tag Protein (Catalog # 11241-OX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 17-34 kDa.

Reconstitution Calculator

Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1¬23) and a carboxy¬terminal extracellular domain (aa 51-183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similarly to other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40 Ligand is one of the co¬stimulatory molecules in the immune system that includes B7, CD40 Ligand, CD30 Ligand, CD27 Ligand and 4¬1BB Ligand. Because OX40 appears as a late activation-induced T cell surface antigen, it has been speculated that the major function of OX40-OX40L interaction is to transmit a late co-stimulatory signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.

References

Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757
Baum, P.R. et al. (1994) EMBO J. 13:3992.
AlShamkhani, A. et al. (1997) J. Biol. Chem. 272:5275.
Kjaergaard, J. et al. (2000) Cancer Res. 60:5514.

Entrez Gene IDs

7292 (Human); 22164 (Mouse); 89814 (Rat)

Alternate Names

CD134 ligand; CD134L; CD252 antigen; CD252; Glycoprotein Gp34; gp34; OX40 antigen ligand; OX40 Ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1, 34kD; TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand superfamily member 4; TXGP1