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Recombinant Human P-Selectin/CD62P Protein, CF

Catalog #: ADP3
17 Citations 1 Review Datasheet / COA / SDS
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ADP3-050
ADP3-200
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Citations (17)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human P-Selectin/CD62P Protein, CF Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to human sP-Selectin coated plates (10 μg/mL with 100 μL/well), >50% will adhere after 1 hour incubation at room temperature.
Source
Chinese Hamster Ovary cell line, CHO-derived human P-Selectin/CD62P protein
Trp42-Ala771
Accession #
P16109
N-terminal Sequence
Analysis
Trp42
Predicted Molecular Mass
80 kDa
SDS-PAGE
121-124 kDa, reducing conditions

Product Datasheets

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Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

ADP3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
Reconstitution Reconstitute at 1 mg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: P-Selectin/CD62P

Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L Selectin,P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble P-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of P-Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

Entrez Gene IDs
6403 (Human); 20344 (Mouse)
Alternate Names
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP

Citations for Recombinant Human P-Selectin/CD62P Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Single cell autofluorescence imaging reveals immediate metabolic shifts of neutrophils with activation across biological systems
    Authors: Datta, R;Miskolci, V;Gallego-López, GM;Britt, E;Gillette, A;Kralovec, A;Giese, MA;Qian, T;Fan, J;Huttenlocher, A;Skala, MC;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  2. P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression
    Authors: E Yeini, P Ofek, S Pozzi, N Albeck, D Ben-Shusha, G Tiram, S Golan, R Kleiner, R Sheinin, S Israeli Da, S Reich-Zeli, R Grossman, Z Ram, H Brem, TM Hyde, P Magod, D Friedmann-, A Madi, R Satchi-Fai
    Nature Communications, 2021-03-26;12(1):1912.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. L-Arginine Modulates Neonatal Leukocyte Recruitment in a Gestational Age-Dependent Manner
    Authors: R Fitterer, T Lajqi, SA Kranig, M Braun, N Theissig, N Kuss, J Pöschl, D Frommhold, H Hudalla
    J Clin Med, 2020-08-27;9(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Platelets Fuel the Inflammasome Activation of Innate Immune Cells
    Authors: V Rolfes, LS Ribeiro, I Hawwari, L Böttcher, N Rosero, S Maasewerd, MLS Santos, T Próchnicki, CMS Silva, CWS Wanderley, M Rothe, SV Schmidt, HJ Stunden, D Bertheloot, MN Rivas, CJ Fontes, LH Carvalho, FQ Cunha, E Latz, M Arditi, BS Franklin
    Cell Rep, 2020-05-12;31(6):107615.
    Species: Human
    Sample Types:
    Applications: Cell Culture
  5. Glycosaminoglycans as Tools to Decipher the Platelet Tumor Cell Interaction: A Focus on P-Selectin
    Authors: S Schwarz, LM Gockel, A Naggi, U Barash, M Gobec, G Bendas, M Schlesinge
    Molecules, 2020-02-26;25(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. The P-selectin and PSGL-1 axis accelerates atherosclerosis via activation of dendritic cells by the TLR4 signaling pathway
    Authors: Z Ye, L Zhong, S Zhu, Y Wang, J Zheng, S Wang, J Zhang, R Huang
    Cell Death Dis, 2019-07-01;10(7):507.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Neutrophil activation and NETosis are the major drivers of thrombosis in heparin-induced thrombocytopenia
    Authors: J Perdomo, HHL Leung, Z Ahmadi, F Yan, JJH Chong, FH Passam, BH Chong
    Nat Commun, 2019-03-21;10(1):1322.
    Species: Human
    Sample Types: Whole Blood
    Applications: Bioassay
  8. L-selectin expression is regulated by CXCL8-induced reactive oxygen species produced during human neutrophil rolling
    Authors: MJ Domínguez-, E Armas-Gonz, A Herrera-Ga, M Arce-Franc, M Feria, M Vicente-Ma, A Martínez-R, F Sánchez-Ma, F Díaz-Gonzá
    Eur. J. Immunol., 2018-12-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity
    Sci Rep, 2016-11-28;6(0):37953.
    Applications: ELISA Developmet
  10. Platelets Guide Leukocytes to Their Sites of Extravasation
    PLoS Biol, 2016-05-06;14(5):e1002459.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  11. Endocarditis pathogen promotes vegetation formation by inducing intravascular neutrophil extracellular traps through activated platelets.
    Authors: Jung C, Yeh C, Hsu R, Lee C, Shun C, Chia J
    Circulation, 2014-12-19;131(6):571-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Saikosaponin D isolated from Bupleurum falcatum inhibits selectin-mediated cell adhesion.
    Authors: Jang M, Kim Y, Bae E, Oh T, Choi H, Lee J, Oh H, Lee S
    Molecules, 2014-12-04;19(12):20340-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. VLA-4 blockade promotes differential routes into human CNS involving PSGL-1 rolling of T cells and MCAM-adhesion of TH17 cells.
    Authors: Schneider-Hohendorf T, Rossaint J, Mohan H, Boning D, Breuer J, Kuhlmann T, Gross C, Flanagan K, Sorokin L, Vestweber D, Zarbock A, Schwab N, Wiendl H
    J Exp Med, 2014-08-18;211(9):1833-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. RAGE and ICAM-1 cooperate in mediating leukocyte recruitment during acute inflammation in vivo.
    Authors: Frommhold D, Kamphues A, Hepper I, Pruenster M, Lukic IK, Socher I, Zablotskaya V, Buschmann K, Lange-Sperandio B, Schymeinsky J, Ryschich E, Poeschl J, Kupatt C, Nawroth PP, Moser M, Walzog B, Bierhaus A, Sperandio M
    Blood, 2010-04-20;116(5):841-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Regulation of leukocyte recruitment by the long pentraxin PTX3.
    Authors: Deban L, Russo RC, Sironi M, Moalli F, Scanziani M, Zambelli V, Cuccovillo I, Bastone A, Gobbi M, Valentino S, Doni A, Garlanda C, Danese S, Salvatori G, Sassano M, Evangelista V, Rossi B, Zenaro E, Constantin G, Laudanna C, Bottazzi B, Mantovani A
    Nat. Immunol., 2010-03-07;11(4):328-34.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  16. Capture and enrichment of CD34-positive haematopoietic stem and progenitor cells from blood circulation using P-selectin in an implantable device.
    Authors: Wojciechowski JC, Narasipura SD, Charles N, Mickelsen D, Rana K, Blair ML, King MR
    Br. J. Haematol., 2008-01-22;140(6):673-81.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  17. Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling.
    Authors: Bestebroer J, Poppelier MJ, Ulfman LH, Lenting PJ, Denis CV, van Kessel KP, van Strijp JA, de Haas CJ
    Blood, 2007-04-01;109(7):2936-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human P-Selectin/CD62P Protein, CF
By Kyle Blackshere on 07/29/2016
Application: In vivo study