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Recombinant Human p23/PTGES3 Protein, CF

Catalog #: AP-170
2 Citations Datasheet / COA / SDS

Discontinued Product

AP-170 has been discontinued.
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Recombinant Human p23/PTGES3 Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity

p23/PTGES3 can serve as a molecular co-chaperone for HSP90.   Reaction conditions will need to be optimized for each specific application.  For in vitro reactions containing both HSP90 alpha /HSP90AA1 (Catalog # AP-160) and p23/PTGES3, we recommend an initial HSP90 concentration of 2-3 μM and an equimolar concentration of p23.

Source
E. coli-derived human p23/PTGES3 protein
Accession # Q15185
Accession #
Q15185
Predicted Molecular Mass
19 kDa

Product Datasheets

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AP-170

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AP-170

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p23/PTGES3

Prostaglandin E Synthase 3 (also known as Cytosolic prostaglandin E2 synthase, Hsp90 co-chaperone,  Progesterone receptor complex p23, Telomerase-binding protein p23, or p23) is a glutathione-dependent enzyme found in the cyclooxygenase-1-mediated PGE2 biosynthetic pathway. In addition to its catalytic activity in the prostaglandin biosynthesis pathway, P23 serves as a co-chaperone to HSP90 in various biological functions. The p23/HSP90 complex is required for efficient telomerase assembly in vitro and in vivo. It has also been demonstrated that p23 and HSP90 localize to genomic response elements in a hormone-dependent manner, and may promote disassembly of transcriptional regulatory complexes in response to changes in cellular signaling pathways. This recombinant protein may be used in conjunction with HSP90 alpha (AP-160) in various in vitro protein refolding assays.

References
  1. Freeman B.C. & Yamamoto K.R. (2002)  Science  296: 2232-2235.
  2. Forsythe H.L., et al. (2001) J. Biol. Chem.  276: 15571-15574.
  3. Patwardhan C.A., et al.  (2013)  J. Biol. Chem.  288: 7313-7325.
Long Name
Prostaglandin E Synthase 3 (Cytosolic)
Entrez Gene IDs
10728 (Human); 56351 (Mouse); 362809 (Rat)
Alternate Names
CPGES; cPGESp23; Cytosolic prostaglandin E2 synthase; Hsp90 co-chaperone; p23; P23cytosolic prostaglandin E synthase; Progesterone receptor complex p23; prostaglandin E synthase 3 (cytosolic); prostaglandin E synthase 3; PTGES3; TEBP; TEBPEC 5.3.99.3; Telomerase-binding protein p23; unactive progesterone receptor, 23 kD

Citations for Recombinant Human p23/PTGES3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The STING ligand cGAMP potentiates the efficacy of vaccine-induced CD8+ T cells
    Authors: A Gutjahr, L Papagno, F Nicoli, T Kanuma, N Kuse, MP Cabral-Pic, N Rochereau, E Gostick, T Lioux, E Perouzel, DA Price, M Takiguchi, B Verrier, T Yamamoto, S Paul, V Appay
    JCI Insight, 2019-04-04;4(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Self-Renewing Trophoblast Organoids Recapitulate the Developmental Program of the Early Human Placenta
    Authors: S Haider, G Meinhardt, L Saleh, V Kunihs, M Gamperl, U Kaindl, A Ellinger, TR Burkard, C Fiala, J Pollheimer, S Mendjan, PA Latos, M Knöfler
    Stem Cell Reports, 2018-08-02;0(0):.
    Species: Human
    Sample Types: Whole Cells

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