Recombinant Human PD-ECGF/Thymidine Phosphorylase Protein
Recombinant Human PD-ECGF/Thymidine Phosphorylase Protein Summary
Product Specifications
Ala11-Gln482
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
229-PE
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
229-PE/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: PD-ECGF/Thymidine Phosphorylase
PD-ECGF, also known as TPase and gliostatin, is produced by placenta, platelets, liver, lung, spleen, lymph nodes, and peripheral lymphocytes. It is overexpressed by many tumors in response to chemical or physical stress. PD-ECGF promotes endothelial cell proliferation and chemotaxis and promotes angiogenesis. PD-ECGF is a key enzyme in the pyrimidine nucleoside salvage pathway. It also metabolizes and inactivates chemotherapeutic drugs such as 5-fluorouracil and its derivatives. The human PD-ECGF cDNA encodes a 482 amino acid (aa) polypeptide with a 10 aa propeptide. The protein is localized mostly within the producer cells. N-terminal truncation, resulting in proteins lacking 10 aa and 6 aa, has been observed in PD-ECGF purified from platelets and placenta, respectively. In solution, PD-ECGF exists as a non-disulfide linked homodimer. The latter protein was purified from quiescent astrocytes and described as an astrocyte and astrocytoma cell growth inhibitor based on its ability to inhibit 3H-thymidine incorporation.
- Haraguchi, M. et al. (1994) Nature 368:198.
- Toi, M. et al. (2005) Lancet Oncol. 6:158.
- Akiyama, S. et al. (2004) Canc. Sci. 95:851.
Citations for Recombinant Human PD-ECGF/Thymidine Phosphorylase Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Glucocorticoids Suppress Mitochondrial Oxidant Production via Upregulation of Uncoupling Protein 2 in Hyperglycemic Endothelial Cells
PLoS ONE, 2016-04-29;11(4):e0154813.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Regulation of vascular endothelial growth factor expression in human colon cancer by insulin-like growth factor-I.
Authors: Akagi Y, Liu W, Zebrowski B, Xie K, Ellis LM
Cancer Res., 1998-09-01;58(17):4008-14.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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