Recombinant Human PGLYRP4/PGRP-I beta Protein, CF

Catalog #: 8389-PG Datasheet / COA / SDS

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8389-PG-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human PGLYRP4/PGRP-I beta Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When peptidoglycan is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human PGLYRP4/PGRP-I beta that produces 50% optimal binding response is 0.5-3 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human PGLYRP4/PGRP-I beta protein
Asp18-His373 with a C-terminal 6-His tag

Accession #

AAK72485

N-terminal Sequence
Analysis

Asp18

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

40 kDa

SDS-PAGE

53-64 kDa, reducing conditions

Product Datasheets

8389-PG

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8389-PG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: PGLYRP4/PGRP-I beta

PGLYRP4, also known as PGRP-I beta, is a member of the peptidoglycan recognition protein family of innate immunity proteins (1, 2). Human PGLYRP4 is expressed in the skin, eyes, salivary glands, throat, tongue, esophagus, stomach, and intestine (3). Mature human PGLYRP4 contains two nonidentical PGRP domains, and it shares 77% and 74% amino acid sequence identity with mouse and rat PGLYRP4, respectively (1, 4). It is secreted as disulfide-linked homodimers and binds peptidoglycan (PGN) and PGN-containing Gram-positive bacteria (1, 3). PGLYRP4 is directly bactericidal against pathogenic and nonpathogenic Gram-positive bacteria, but not normal flora bacteria, suggesting that normal flora bacteria have developed resistance to this bactericidal mechanism (3, 5, 6). Its bactericidal activity requires physiological concentrations of Zn²⁺ (6). PGLYRP4 knockout mice are more sensitive to the development of experimental dermatitis and DSS-induced colitis than wild type mice (2, 7). In humans, PGLYRP4 single nucleotide polymorphisms have been associated with inflammatory bowel disease and increased Parkinson’s disease risk (8, 9).

References