Recombinant Human PILR-beta Fc Chimera Avi-tag Protein, CF

Catalog #: AVI9828 Datasheet / COA / SDS

Biotinylated

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AVI9828-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human PILR-beta Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CL-P1/COLEC12 (Catalog # 2690-CL) is immobilized at 2 μg/mL, 100 μg/mL, Biotinylated Recombinant Human PILR‑ beta Fc Chimera Avi-tag binds with an ED₅₀ of 0.600-6.00 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human PILR-beta protein

Human PILR- beta (Gln20-Ala189) Accession # Q9UKJ0.1	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

Q9UKJ0.1

N-terminal Sequence
Analysis

Gln20, as confirmed by mass spectrometry.

Structure / Form

Biotinylated via Avi-tag.

Predicted Molecular Mass

48 kDa

SDS-PAGE

55-68 kDa, under reducing conditions.

Product Datasheets

AVI9828

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI9828

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human CL-P1/COLEC12 (2690-CL) is immobilized at 2 μg/mL, 100 μg/mL, Biotinylated Recombinant Human PILR‑ beta Fc Chimera Avi-tag (Catalog # AVI9828) binds with an ED₅₀ of 0.600-6.00 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human PILR-beta Fc Chimera Avi-tag Protein (Catalog # AVI9828) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-68 kDa.

Reconstitution Calculator

Background: PILR-beta

Paired immunoglobulin-like type 2 receptor beta (PILR-beta ) is a type I transmembrane (TM) glycoprotein that belongs to the Ig superfamily (1). PILR-beta is the activating counterpart to the inhibitory immunoreceptor PILR-alpha, forming a set of paired immune regulatory receptors responsible for regulating inflammatory responses (1). Mature human PILR-beta contains a V-type Ig-like extracellular domain (ECD) with a siglec-like fold, a single transmembrane domain, and a truncated cytoplasmic tail (2, 3). The transmembrane domain of PILR-beta contains a positively charged residue which interacts with immunoreceptor tyrosine-based activation (ITAM)-bearing adaptor molecules (2). The ECD of mature human PILR-beta shares 40% aa sequence identity with the ECD of mouse PILR-beta. PILR-beta is expressed on myeloid cells, such as natural killer, macrophage, and dendritic cells, as well as resident cells of the central nervous system, such as microglial cells (2,4). It is a binding partner for DAP12 and CD99, and has been shown to play an important role in innate immunity and inflammation (4-6). The PILR-alpha / beta pair have also been shown to regulate cell signaling via association with SHP-1 (7). Experiments studying the effects of S. aureus and T. gondii infections in mice have shown that up-regulation of PILR-beta led to significantly lower survival rates while knock-down of PILR-beta or activation of PILR-alpha resulted in significantly less inflammation and increased pathogen clearance (4,5). Our Avi-tag Biotinylated PILR-beta features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Wilson, M.D. et al. (2006) Physiol. Genomics 27:201.
Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
Lu, Q. et al. (2014) PNAS 111:8221.
Tato, C.M. et al. (2012) PLoS One 7:e31690.
Banerjee, A. et al. (2010) Infect. Immun. 78:1353.
Tabata, S. et al. (2008) J. Biol. Chem. 283:8893.
Mousseau, D.D. et al. (2000) J. Biol. Chem. 275:4467

Long Name

Paired-Ig-likeType 2 Receptor beta

Entrez Gene IDs

29990 (Human); 170741 (Mouse)

Alternate Names

activating receptor PILRbeta; Activating receptor PILR-beta; Cell surface receptor FDFACT; cell surface receptor FDFACT1; cell surface receptor FDFACT2; FDFACT; FDFACT1; FDFACT2; paired immunoglobin-like receptor beta; paired immunoglobin-like type 2 receptor beta; paired immunoglobulin-like receptor beta; paired immunoglobulin-like type 2 receptor beta; PILRB; PILRbeta; PILR-beta