Recombinant Human PILR-beta Fc Chimera Protein, CF

Catalog #: 9828-PR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9828-PR-050

Recombinant Human PILR-beta Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human PILR-beta Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Recombinant Human CL-P1/COLEC12 (Catalog # 2690-CL) immobilized at 2 μg/mL, 100 μg/mL, can bind to Recombinant Human PILR‑ beta Fc Chimera with an ED₅₀ of 0.12-0.72 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human PILR-beta protein

Human PILR-beta (Gln20-Ala189) Accession # Q9UKJ0-1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q9UKJ0-1

N-terminal Sequence
Analysis

No results obtained. Gln20 predicted.

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

45.7 kDa (monomer)

SDS-PAGE

57-66 kDa, reducing conditions

Product Datasheets

9828-PR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9828-PR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Human CL-P1/COLEC12 (Catalog # 2690-CL) is coated at 2 µg/mL, Recombinant Human PILR-beta Fc Chimera (Catalog # 9828-PR) binds with an ED₅₀ of 0.12-0.72 µg/mL.

Reconstitution Calculator

Background: PILR-beta

Paired immunoglobulin-like type 2 receptor beta (PILR-beta ) is a type I transmembrane (TM) glycoprotein belonging to the Ig superfamily. PILR-beta is the activating counterpart to the immunoreceptor tyrosine-based inhibitory motif (ITIM) containing PILR-alpha inhibitory receptor (1). Mature human PILR-beta is a 208 amino acid (aa) protein containing a 172 aa V-type Ig-like extracellular domain (ECD) with a siglec-like fold, a single TM, and a truncated cytoplasmic tail (2, 3). The TM of PILR-beta contains a positively-charged residue which interacts with immunoreceptor tyrosine-based activation (ITAM)-bearing adaptor molecules (2). The ECD of mature human PILR-beta shares 40% aa sequence identity with its mouse counterpart. PILR-beta is expressed on myeloid cells, such as natural killer, macrophage, and dendritic cells, as well as resident cells of the central nervous system, such as microglial cells (2,4). It is a binding partner for DAP12 and CD99, and has been shown to play an important role in innate immunity and inflammation (4-6). The PILR-alpha / beta pair have also been shown to regulate cell signaling via association with SHP-1 (7). Experiments studying the effects of S. aureus and T. gondii infections in mice have shown that up-regulation of PILR-beta led to significantly lower survival rates while knock-down of PILR-beta or activation of PILR-alpha resulted in significantly less inflammation and increased pathogen clearance (4,5).

References

Wilson, M.D. et al. (2006) Physiol. Genomics 27:201.
Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
Lu, Q. et al. (2014) PNAS 111:8221.
Tato, C.M. et al. (2012) PLoS One 7:e31690.
Banerjee, A. et al. (2010) Infect. Immun. 78:1353.
Tabata, S. et al. (2008) J. Biol. Chem. 283:8893.
Mousseau, DD. et al. (2000) J. Biol. Chem. 275:4467.

Long Name

Paired-Ig-likeType 2 Receptor beta

Entrez Gene IDs

29990 (Human); 170741 (Mouse)

Alternate Names

activating receptor PILRbeta; Activating receptor PILR-beta; Cell surface receptor FDFACT; cell surface receptor FDFACT1; cell surface receptor FDFACT2; FDFACT; FDFACT1; FDFACT2; paired immunoglobin-like receptor beta; paired immunoglobin-like type 2 receptor beta; paired immunoglobulin-like receptor beta; paired immunoglobulin-like type 2 receptor beta; PILRB; PILRbeta; PILR-beta