Recombinant Human Plexin A1 His-tag Protein, CF

Catalog #: 10167-PA Datasheet / COA / SDS

Analyzed by SEC-MALS

Catalog #	Availability	Size / Price	Qty
10167-PA-050

Recombinant Human Plexin A1 His-tag Protein SEC-MALS.

2 Images

Product Details

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Plexin A1 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin A1 His-tag is immobilized at 2.50 µg/mL (100 µL/well), Recombinant Human Semaphorin 6C Fc Chimera (Catalog # 2219-S6) binds with an ED₅₀ of 1.00-6.00 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human Plexin A1 protein
Glu27 - Pro1244, with a C-terminal 6-His tag

Accession #

Q9UIW2.3

N-terminal Sequence
Analysis

Glu27

Predicted Molecular Mass

135 kDa

SDS-PAGE

130-150 kDa, under reducing conditions

Product Datasheets

10167-PA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10167-PA

Formulation	Supplied as a 0.2 μm filtered solution in PBS.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

SEC-MALS

View Larger

Recombinant Human PLXNA-1/C-His (Catalog # 10167-PA) has a molecular weight (MW) of 156.0 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

SDS-PAGE

View Larger

2 μg/lane of Recombinant Human Plexin A1 His-tag (Catalog # 10167-PA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 130-150 kDa...

Reconstitution Calculator

Background: Plexin A1

Plexin A1, also known as Plexin 1, is a type I transmembrane protein that is a member of the Plexin family of Semaphorin signal transducers (1). In humans, the Plexin family consists of at least nine members divided into four subfamilies and Plexin signaling is involved in neuronal axon guidance, angiogenesis and immune response (2). Mature human Plexin A1 consists of an extracellular domain (ECD) with one Sema domain, a spacer, and four tandem IPT/TIG domains, a transmembrane segment, and a large cytoplasmic domain. Within the ECD, human Plexin A1 shares 95% amino acid sequence identity with mouse and rat Plexin A1. Plexin A1 binds Class 3 (secreted) Semaphorins indirectly via Neuropilin (Npn)-1 and Npn-2 and binds transmembrane Semaphorin 6D directly (3 ‑ 5). Sema3A engagement of Plexin A1 and Npn-1 guides proprioceptive and sensory neurons during development, while Sema3B engagement guides floorplate neurons (5-8). In contrast, T cell Sema6D engagement of dendritic cell Plexin A1 controls actin polymeration, which supports formation of immunological synapses and enhances the function of the dendritic cells (3, 4, 9). Complex formation with DAP12 allows Plexin A1 signaling through TREM family proteins (10, 11). However, the most striking effect of Plexin A1 deletion is on bone homeostasis, where Plexin A1-deficient mice show increased trabecular bone mass due to downregulated osteoclast differentiation (10). Plexin A1 and Sema6D are frequently expressed in malignant pleural mesothelioma, where they promote anchorage-independent growth through complexing with and activating VEGF R2 (12). Plexin A1 has also been identified as a receptor for Slits and mediate commissural growth cone collapse (13).

References