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Recombinant Human Podoplanin Fc Chimera Protein, CF

Catalog #: 3670-PL
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
3670-PL-050
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Citations (4)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Podoplanin Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Podoplanin Fc Chimera (Catalog # 3670-PL) can bind Recombinant Human CLEC‑2 (Catalog # 1718-CL) with an estimated Kd <4 nM.
Source
Mouse myeloma cell line, NS0-derived human Podoplanin protein
Human Podoplanin
(Glu97–Lys199) &
(Ala99–Lys199)
Accession # NP_006465.3		 IEGRMD Human IgG1
(Pro100–Lys330)
N-terminus C-terminus
Accession #
NP_006465.3
N-terminal Sequence
Analysis
Glu97 & Ala99
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
37.1 kDa
SDS-PAGE
60-65 kDa, under reducing conditions

Product Datasheets

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3670-PL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3670-PL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Podoplanin

Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family (1-2). Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation (2-3). There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells (4-5), and numerous tumors, including colorectal tumors (3), squamous cell carcinomas (4, 6), testicular seminoma (7), and brain tumors (8-10). One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver (1). Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2) (2). Their association is dependent on sialic acid on O-glycans of Podoplanin (2). Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis (2). Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth (2).

References
  1. Zimmer, G. et al. (1999) Biochem. J. 341:277.
  2. Katsue-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
  3. Kato, Y. et al. (2003) J. Biol. Chem. 278:51599.
  4. Schacht, V. et al. (2005) Am. J. Pathol. 166:913.
  5. Breiteneder-Geleff, S. et al. (1997) Am. J. Pathol. 151:1141.
  6. Kato, Y. et al. (2005) Tumour Biol. 26:195.
  7. Kato, Y. et al. (2004) Oncogene 23:8552.
  8. Mishima, K. et al. (2006) Acta Neuropathol. 111:563.
  9. Mishima, K. et al. (2006) Acta Neuropathol. 111:483.
  10. Kato, Y. et al. (2006) Biochem. Biophys. Res. Commun. 349:1301.
Long Name
Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A
Entrez Gene IDs
10630 (Human); 14726 (Mouse); 54320 (Rat)
Alternate Names
36-KD; Aggrus; Gp36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTS 8; OTS8; PA2.26 antigen; PDPN; Podoplanin; RANDAM-2; T1A; T1A-2; T1-alpha

Citations for Recombinant Human Podoplanin Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Polysaccharide-containing fraction from Artemisia argyi inhibits tumor cell-induced platelet aggregation by blocking interaction of podoplanin with C-type lectin-like receptor 2
    Authors: CP Tseng, YL Huang, YW Chang, HR Liao, YL Chen, PW Hsieh
    J Food Drug Anal, 2019-09-26;28(1):115-123.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. IGF-1R deficiency in human keratinocytes disrupts epidermal homeostasis and stem cell maintenance
    Authors: T Muraguchi, D Nanba, EK Nishimura, T Tashiro
    J. Dermatol. Sci., 2019-05-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Inhibitory effects of polypeptides derived from a snake venom C-type lectin, aggretin, on tumor cell-induced platelet aggregation.
    Authors: Chang C, Chung C, Hsu C, Peng H, Huang T
    J Thromb Haemost, 2014-04-01;12(4):540-9.
    Applications: Bioassay
  4. Platelets regulate lymphatic vascular development through CLEC-2-SLP-76 signaling.
    Authors: Bertozzi CC, Schmaier AA, Mericko P
    Blood, 2010-04-02;116(4):661-70.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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