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Recombinant Human R-Spondin 4 Protein

Catalog #: 4575-RS
8 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
4575-RS-025/CF

With Carrier

Catalog # Availability Size / Price Qty
4575-RS-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (8)
FAQs
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human R-Spondin 4 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 10‑60 ng/mL in the presence of 10 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Source
Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 4 protein
Met1-Pro234
Accession #
Q2I0M5
N-terminal Sequence
Analysis
Ala19
Predicted Molecular Mass
24.1 kDa (monomer)
SDS-PAGE
30-36 kDa, reducing conditions

Product Datasheets

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4575-RS (with carrier)

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4575-RS/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4575-RS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

4575-RS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: R-Spondin 4

R-Spondin 4 (RSPO4, roof plate-specific spondin 4), also called cysteine‑rich and single thrombospondin domain containing-4 (Cristin 4), is an ~33 kDa secreted heparin-binding protein that shares ~35% amino acid (aa) identity with three other R‑Spondin family members (1‑3). All are positive modulators of Wnt/ beta -catenin signaling, but vary in activity (2). R‑Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK-1-mediated internalization (1, 4). Like other R‑spondins, human R‑Spondin 4 (228 aa) contains a signal sequence (aa 1‑19), two adjacent cysteine‑rich furin-like domains (aa 85-128) with one potential tyrosine phosphorylation site (aa 114), followed by a thrombospondin (TSP‑1) motif (aa 137‑197) and a region rich in basic residues (aa 199‑228). Mature human R‑Spondin 4 shares 81%, 81%, 84%, 84% and 86% aa identity with mouse, rat, equine, canine and bovine R‑Spondin 4, respectively. Of two potential isoforms, one lacks the TSP-1 domain, while another terminates at aa 224 (5). Each R‑Spondin has a distinct expression pattern (6). In the mouse, R‑Spondin 4 mRNA is found during development of limb bud mesenchyme, nail beds, heart and teeth (6‑8). In humans, mutations of R‑Spondin 4 have been found to cause anonychia, a condition in which fingernails and toenails are absent (8‑10).

References
  1. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  2. Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588.
  3. Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229.
  4. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
  5. Entrez Accession # NP_001035096, EAX10652.
  6. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
  7. Pemberton, T.J. et al. (2007) Dev. Dyn. 236:2245.
  8. Ishii, Y. et al. (2008) J. Invest. Dermatol. 128:867.
  9. Blaydon, D.C. et al. (2006) Nat. Genet. 38:1245.
  10. Bergmann, C. et al. (2006) Am. J. Hum. Genet. 79:1105.
Long Name
Roof Plate-specific Spondin 4
Entrez Gene IDs
343637 (Human); 228770 (Mouse)
Alternate Names
C20orf182; chromosome 20 open reading frame 182; Cristin 4; CRISTIN4; dJ824F16.3; FLJ16018; hRspo4; Roof plate-specific spondin-4; RSPO4; RSpondin 4; R-Spondin 4; R-spondin family, member 4; R-spondin-4

Citations for Recombinant Human R-Spondin 4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Hyperglycemia increases SCO-spondin and Wnt5a secretion into the cerebrospinal fluid to regulate ependymal cell beating and glucose sensing
    Authors: Nualart, F;Cifuentes, M;Ramírez, E;Martínez, F;Barahona, MJ;Ferrada, L;Saldivia, N;Bongarzone, ER;Thorens, B;Salazar, K;
    PLoS biology
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Dot Blot
  2. R-spondins are BMP receptor antagonists in Xenopus early embryonic development
    Authors: H Lee, C Seidl, R Sun, A Glinka, C Niehrs
    Nat Commun, 2020-11-04;11(1):5570.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Differential activities and mechanisms of the four R-Spondins in potentiating Wnt/?-catenin signaling
    Authors: S Park, J Cui, WA Yu, L Wu, K Carmon, QJ Liu
    J. Biol. Chem., 2018-05-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. R-spondins can potentiate WNT signaling without LGRs
    Authors: AM Lebensohn, R Rohatgi
    Elife, 2018-02-06;7(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling.
    Authors: Carmon K, Gong X, Yi J, Thomas A, Liu Q
    Proc Natl Acad Sci U S A, 2014-03-17;111(13):E1221-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer.
    Authors: Wu C, Qiu S, Lu L, Zou J, Li W, Wang O, Zhao H, Wang H, Tang J, Chen L, Xu T, Sun Z, Liao W, Luo G, Lu X
    Nat Commun, 2014-01-01;5(0):3149.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner.
    Authors: Hao HX, Xie Y, Zhang Y, Charlat O, Oster E, Avello M, Lei H, Mickanin C, Liu D, Ruffner H, Mao X, Ma Q, Zamponi R, Bouwmeester T, Finan PM, Kirschner MW, Porter JA, Serluca FC, Cong F
    Nature, 2012-04-29;485(7397):195-200.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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