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Recombinant Human RAP80 UIM Domains Biotin Protein, CF

Catalog #: UBE-235 Datasheet / COA / SDS

Discontinued Product

UBE-235 has been discontinued.
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Recombinant Human RAP80 UIM Domains Biotin Protein, CF Summary

Specifications

Source
E. coli
Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Applications
Enzyme Activity
Species
Human

Product Datasheets

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COA
SDS

Background: RAP80

Rap80 (Receptor Associated Protein 80) interacts with BRCA1, a ubiquitin E3-ligase which functions in conjugation with the BARD1 deubiquitinating enzyme. BRCA1 is recruited to DNA damage sites by poly-ubiquitin chains through Rap80 which contains 2 tandem ubiquitin-interacting motifs (UIMs). Rap80 constitutes a protein complex with ABRA1 which interacts with the BRCT domain of BRCA1. Upon DNA damage the Rap80-ABRA1 complex targets the BRCA1-BARD1 complex to K6- and K63-linked poly-Ub chains at these foci. The UIM domains of Rap80 have been shown to have preferential binding to K-6 and K63-linked Ub chains and binds to K48-chain with a much lower efficiency. It is not known if Rap80 UIMs interact with Ub chains linked via K11, K27 or K33. Rap80 has a low affinity for mono-, di- and tri-Ub but binds efficiently to tetra (or greater) ubiquitin chains. Detection with avidin-linked reagents allows for a higher efficiency and detection sensitivity than with other antibodies.

References
  1. Buchberger A. (2002) Trends Cell. Biol. 12 :216-221
  2. Hurley J. H. et al. (2006) Biochem. J. 399 :361-372
  3. Kim H. et al. (2007) Science 316:1202-1205
  4. Kim H. et all. (2008) Mol. Cell. 25:457-61
  5. Sobhian B. et all. (2007) Science 316:1198-1202
  6. Wang B. (2007) Science 316:1194-1198
  7. Wu W. et al. (2008) Cell Div. 31-10
  8. Yan J. et al. (2007) Canc. Res. 15:6647-6656
Long Name
Receptor Associated Protein 80
Entrez Gene IDs
51720 (Human); 20184 (Mouse); 290997 (Rat)
Alternate Names
BRCA1-A complex subunit RAP80; RAP80; RAP80X2HRIP110; receptor associated protein 80; Receptor-associated protein 80; retinoid x receptor interacting protein; Retinoid X receptor-interacting protein 110; RXRIP110; ubiquitin interaction motif containing 1; Ubiquitin interaction motif-containing protein 1; UIMC1; X2HRIP110

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