Recombinant Human ROR1 Fc Chimera Protein, CF

Catalog #: 9490-RO Datasheet / COA / SDS

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9490-RO-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ROR1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Measured by its ability to bind biotinylated recombinant human/mouse Wnt-5a in a functional ELISA. The ED₅₀ for this effect is 8-48 µg/mL.

Source

Human embryonic kidney cell, HEK293-derived human ROR1 protein

Human ROR1 (Ala24-Glu403) Accession # Q01973-1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q01973-1

N-terminal Sequence
Analysis

Ala24

Structure / Form

Disulfide -linked homodimer

Predicted Molecular Mass

69 kDa

SDS-PAGE

84-97 kDa, reducing conditions

Product Datasheets

9490-RO

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9490-RO

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: ROR1

ROR1 (Receptor tyrosine kinase-like orphan receptor 1), also known as neurotrophic tyrosine kinase receptor-related 1 (NTRKR1), is a member of the ROR family within receptor tyrosine kinases (RTK) superfamily. Two ROR family members (ROR1 and ROR2) have been identified and are characterized by the intracellular tyrosine kinase domains, highly related to those of the Trk-family receptor tyrosine kinases, and by the extracellular Frizzled-like cysteine-rich domains and kringle domains, which are common to receptors of the Wnt family members (1, 2). Human ROR1 is a type I transmembrane protein with 937 amino acids (aa) in length. It contains a 29 aa signal sequence, a 377 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 510 aa cytoplasmic region. Human ROR1 shares 97% and 58% aa sequence identity with mouse ROR1 and human ROR2, respectively (2, 3). ROR1 has been shown to play crucial roles in developmental morphogenesis by acting as receptors or co-receptors to mediate Wnt5a-induced signaling (4). The bioactivity of ROR1 is measured by its ability to bind biotinylated recombinant mouse Wnt-5a in a functional ELISA.

References

Aghebati-Maleki, L. et al. (2017) Biomed. Pharmacother. 88:814.
Borcherding N. et al. (2014) Protein Cell. 5:496.
Masiakowski P. and Carroll R.D. (1992) J. Biol. Chem. 267:26181.
Fukuda T. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:3047.

Long Name

Receptor Tyrosine Kinase-like Orphan Receptor 1

Entrez Gene IDs

4919 (Human); 26563 (Mouse)

Alternate Names

EC 2.7.10.1; MGC99659; neurotrophic tyrosine kinase receptor-related 1; Neurotrophic tyrosine kinase, receptor-related 1; NTRKR1; NTRKR1dJ537F10.1; receptor tyrosine kinase-like orphan receptor 1; ROR1; tyrosine-protein kinase transmembrane receptor ROR1