Recombinant Human SECTM1 Fc Chimera Protein, CF

Catalog #: 7559-ST Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7559-ST-050

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human SECTM1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 5 x 10⁴cells per well are added to Recombinant Human SECTM1 Fc Chimera coated plates, cell adhesion is enhanced in a dose dependent manner. The ED₅₀ for this effect is 1.5-7.5 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Source

Chinese Hamster Ovary cell line, CHO-derived human SECTM1 protein

Human SECTM1 (Gln29-Gly145) Accession # Q8WVN6	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q8WVN6

N-terminal Sequence
Analysis

Gln29 predicted: No results obtained, sequencing might be blocked

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

39.3 kDa (monomer)

SDS-PAGE

50-60 kDa, reducing conditions

Product Datasheets

7559-ST

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7559-ST

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: SECTM1

SECTM1 (secreted and transmembrane 1), also called K12, is either found as an approximately 27 kDa intracellular type I transmembrane protein that shows a perinuclear, Golgi‑like staining pattern, or as a 20 kDa soluble, secreted form (1, 2). SECTM1 protein expression is detected in some myeloid cells, such as stimulated monocytes, immature monocyte‑derived dendritic cells, and granulocytes (1‑3). In the thymus, it is also expressed by epithelia and fibroblasts (2). Stimulation with IFN‑ gamma is often necessary to detect SECTM1 expression, and it is thought to be an interferon early‑response gene (1‑5). Human SECTM1 cDNA encodes 248 amino acids (aa), including a 28 aa signal sequence, a 117 aa extracellular domain (ECD) with one potential N‑linked glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic sequence (1). The ECD shows some similarity to Ig domains (1). The human SECTM1 ECD shares limited (approximately 45‑51%) aa sequence identity with mouse SECTM1 (a or b) and rat SECTM1 ECD. SECTM1 from human and mouse each show species‑specific binding of CD7 and co‑stimulate CD4⁺ and CD8⁺ T cells through CD7, either alone or through synergy with CD28 (2‑4). Immobilized recombinant human SECTM1/Fc also enhances human NK cell expression of activation markers such as CD25, CD69 and CD54 (4).

References