Recombinant Human Semaphorin 3G Fc Chimera Protein, CF

Catalog #: 10804-S3 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10804-S3-025

Recombinant Human Semaphorin 3G Fc Chimera Protein Bioactivity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Semaphorin 3G Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability to inhibit the proliferation of HUVECumbilical vein endothelial cells.
The ED₅₀ for this effect is 100-600 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human Semaphorin 3G protein

Human Semaphorin 3G (Gly23-Thr782)(R557S, R558S, R560S, R561S, R774S, R777S) Accession # Q9NS98.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q9NS98.1

N-terminal Sequence
Analysis

Gly23

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

111 kDa

SDS-PAGE

105-125 kDa, under reducing conditions

Product Datasheets

10804-S3

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10804-S3

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human Semaphorin 3G Fc Chimera inhibits the proliferation of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 100-600 ng/mL.

Reconstitution Calculator

Background: Semaphorin 3G

Semaphorin 3G (Sema3G), also known as Semaphorin sem2, is a class 3 member of the semaphorin family initially identified as regulators of neuron-axonal guidance (1, 2). More than 30 semaphorins have been discovered and are divided into eight classes: classes 1 and 4–7 are membrane-associated, while classes 2, 3, and 8 are in a secreted form (1, 2). Mature human Sema3G consists of an N-terminal sema domain, a plexin-semaphorin-integrin (PSI) domain, an Ig-like C2-type domain and a C-terminal basic domain (3). Within the extracellular domain, human Sema3G shares 87% amino acid sequence identity with mouse and rat Sema3G. In vitro analysis shows Sema 3G binds to Neuropilin-2, which forms a receptor complex with Plexin-D1. Via this pathways Sema 3G controls lymphatic vascular patterning by the means of cellular collapse (4). It shows protective effects in ischemic retinopathies by coordinating interactions of beta -catenin and VE-Cadherin in endothelium (5). Sema 3G has been shown to be involved in adipocyte differentiation. Knockdown of Sema3G inhibited weight gain through PI3K/Akt/GSK3 beta signaling in the adipose tissue and the AMPK/SREBP-1c pathway in the liver. Thus, Sema 3G is an adipokine essential for adipogenesis, lipogenesis, and insulin resistance and is associated with obesity (6).

References

Alto, L.T. and Terman, J.R. (2017) Methods Mol. Biol. 1493:1.
Jackson, R.E. and Eickholt, B.J. (2009) Curr. Biol. 19:R504.
Toledano S. et al. (2019) J. Mol. Sci. 20:556.
Liu, X. et al. (2016) Cell Rep. 17:2299.
Chen, D. et al. (2021) J. Clin. Invest. 131:e135296.
Liu, M. et al. (2020) J. Endocrinol. 244:223.

Entrez Gene IDs

56920 (Human); 218877 (Mouse); 290562 (Rat)

Alternate Names

(semaphorin) 3G; FLJ00014; MGC119473; sem2; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; SEMA3G; Semaphorin 3G; Semaphorin sem2; Semaphorin-3G