Recombinant Human Semaphorin 4B Fc Chimera Protein, CF

Catalog #: 10262-S4 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10262-S4-050

Recombinant Human Semaphorin 4B Fc Chimera Protein SDS-PAGE

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Semaphorin 4B Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Semaphorin 4B Fc Chimera (Catalog # 10262-S4), immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.

Source

Mouse myeloma cell line, NS0-derived human Semaphorin 4B protein

Human Semaphorin 4B (Leu44-Glu717) Accession # Q9NPR2.4	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q9NPR2.4

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

101 kDa

SDS-PAGE

105-118 kDa, under reducing conditions

Product Datasheets

10262-S4

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10262-S4

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Human Semaphorin 4B Fc Chimera (Catalog # 10262-S4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-118 kDa and 210-240 kDa, respectively.

Reconstitution Calculator

Background: Semaphorin 4B

Semaphorin 4B (Sema4B) is a 95-100 kDa, class IV member of the Semaphorin family of proteins (1). Mature human Sema4B is a type I transmembrane glycoprotein that is 794 amino acids (aa) in length. It contains a 674 aa extracellular domain (ECD) that is characterized by one Sema domain, a PSI region, and an Ig-like C2-type domain. Within the ECD, human Sema4B shares 85% and 86% aa identity with the mouse and rat Sema4B, respectively (1, 2). Sema4B is expressed in hippocampal neurons, glial cells, and immune cells (3, 4). It colocalizes and interacts with PSD-95 and participates in the formation or functioning of glutamatergic synapses (4). Sema4B^-/- mice display reduced proliferation of astrocytes after CNS injury (5). In the immune system, Sema4B is a negative regulator of basophil-mediated immune response and is implicated in the development of lung cancer (6, 7).

References

Gurrapu, S. et al. (2016) Cell Adh. Migr. 10:675.
Alto, L. and J.R. Terman (2017) Methods. Mol. Biol. 1493:1.
Schultze, W. et al. (2001) J. Neurochem. 78:482.
Burkhardt, C. et al. (2005) FEBS Lett. 579:3821.
Ben-Gigi, L. et al. (2015) eNeuro. 2:1.
Nakagawa, Y. et al. (2011) J. Immunol. 186:2881.
Nagai, H. et al. (2007) Oncogene. 26:4025.

Entrez Gene IDs

10509 (Human); 20352 (Mouse); 293042 (Rat)

Alternate Names

KIAA1745sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4B; MGC131831; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4B; SEMA4B; SEMAC; Semaphorin 4B; semaphorin-4B; SemC