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Recombinant Human sFRP-4 Protein

Catalog #: 1827-SF
9 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
1827-SF-025/CF

With Carrier

Catalog # Availability Size / Price Qty
1827-SF-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human sFRP-4 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 4-20 µg/mL, in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human sFRP-4 protein
Ala22-Val346, with a C-terminal 10-His tag
Accession #
ACC04617
N-terminal Sequence
Analysis
Ala22
Structure / Form
Recombinant Human sFRP-4 is prone to proteolytic cleavage at C-terminus, therefore the poly-His tag at C-terminus may not be present in the preparation.
Predicted Molecular Mass
38.9 kDa
SDS-PAGE
55 kDa, reducing conditions

Product Datasheets

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1827-SF (with carrier)

Product Datasheet
COA
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1827-SF/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1827-SF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1827-SF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: sFRP-4

Secreted Frizzled Related Proteins (sFRPs) are a family of vertebrate proteins which contain homology to the ligand-binding domain of the Frizzled family of transmembrane receptors. sFRPs are approximately 30-35 kDa in size and are comprised of 3 domains: a signal sequence; a cysteine-rich domain (CRD) of about 110 amino acids (aa) with high degree of similarity to the Frizzled proteins, including 10 conserved cysteines; and a 175 aa conserved hydrophilic carboxy terminal region. Because sFRPs contain a CRD very similar to the region responsible for binding Wnt ligands in Frizzleds, sFRPs are thought to act as soluble antagonists of Wnt signals, often postulated to act as tumor suppressor genes (1).

sFRP-4, also known as DDC-4, FrpAP, frpHE and FrzB-2, is expressed in brain, kidney, lung, ovary, prostate, mammary gland, and endometrium (1, 2). This protein shows complex functions with respect to cell survival: it is up‑regulated with apoptosis during ovulation (3), regulates apoptosis in chondrocytes (4), and promotes apoptosis in mammary glands when expressed in transgenic mice (5). On the other hand, sFRP-4 can also act to enhance growth as it is up‑regulated in endometrial and breast carcinomas (6, 7). Since it is not detected in other carcinomas such as the ovary, colon, and pancreas, this suggest that its role in cancer is likely to be tissue dependent (6). In addition sFRP-4 is characterized as a circulating phosphaturic factor expressed by tumors associated with osteomalacia that antagonizes renal Wnt signaling (7). Of all the secreted frizzled related proteins, sFRP-4 is most closely related to sFRP-3 (1). Mouse and human sFRP-4 proteins share 92% aa identity.

References
  1. Jones, S.E. and C. Jomary (2002) Bioessays 24:811.
  2. Wolf, V. et al. (1997) FEBS Letters 417:385.
  3. Drake, J.M. et al. (2003) Apoptosis 8:389.
  4. James, I.E. et al. (2000) Osteoarthritis & Cartilage 8:452.
  5. Lacher, M.D. et al. (2003) Cell Death Differ. 10:528.
  6. Abu-Jawdeh, G. et al. (1999) Lab Investigation 79:439.
  7. Wong, S.C.C. et al. (2002) J. Pathology 196:145.
  8. Berndt, T. et al. (2003) J. Clin. Invest. 112:785.
Long Name
Secreted Frizzled Related Protein 4
Entrez Gene IDs
6424 (Human); 20379 (Mouse); 89803 (Rat)
Alternate Names
DDC-4; Frizzled protein, human endometrium; FRP-4; FRP-AP; frpHE; FRPHEMGC26498; FrzB2; secreted frizzled-related protein 4; secreted frizzled-related protein 4; secreted frizzled-related protein 4; sFRP4; sFRP-4

Citations for Recombinant Human sFRP-4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Phagocytosis of Wnt inhibitor SFRP4 by late wound macrophages drives chronic Wnt activity for fibrotic skin healing
    Authors: D Gay, G Ghinatti, CF Guerrero-J, RA Ferrer, F Ferri, CH Lim, S Murakami, N Gault, V Barroca, I Rombeau, P Mauffrey, L Irbah, E Treffeisen, S Franz, A Boissonnas, C Combadière, M Ito, MV Plikus, PH Romeo
    Sci Adv, 2020-03-20;6(12):eaay3704.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Epigenetic demethylation of sFRPs, with emphasis on sFRP4 activation, leading to Wnt signalling suppression and histone modifications in breast, prostate, and ovary cancer stem cells
    Authors: A Deshmukh, F Arfuso, P Newsholme, A Dharmaraja
    Int. J. Biochem. Cell Biol., 2019-01-30;109(0):23-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Regulation of Cancer Stem Cell Metabolism by Secreted Frizzled-Related Protein 4 (sFRP4)
    Authors: A Deshmukh, F Arfuso, P Newsholme, A Dharmaraja
    Cancers (Basel), 2018-01-31;10(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Differential expression of cell cycle and WNT pathway-related genes accounts for differences in the growth and differentiation potential of Wharton's jelly and bone marrow-derived mesenchymal stem cells
    Authors: AK Batsali, C Pontikoglo, D Koutroulak, KI Pavlaki, A Damianaki, I Mavroudi, K Alpantaki, E Kouvidi, G Kontakis, HA Papadaki
    Stem Cell Res Ther, 2017-04-26;8(1):102.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq
    PLoS ONE, 2016-05-03;11(5):e0154531.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Epigenetic downregulation of SFRP4 contributes to epidermal hyperplasia in psoriasis.
    Authors: Bai J, Liu Z, Xu Z, Ke F, Zhang L, Zhu H, Lou F, Wang H, Fei Y, Shi Y, Wang H
    J Immunol, 2015-03-30;194(9):4185-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways.
    Authors: Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, Hwang S, Renshaw P
    PLoS ONE, 2014-08-29;9(8):e104937.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Wnt3a binds to several sFRPs in the nanomolar range.
    Authors: Wawrzak D, Metioui M, Willems E, Hendrickx M, de Genst E, Leyns L
    Biochem. Biophys. Res. Commun., 2007-04-19;357(4):1119-23.
    Species: Mouse
    Sample Types: Recombinant Protein, Whole Cells
    Applications: Bioassay, Surface Plasmon Resonance

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Recombinant Human sFRP-4 Protein
By Anonymous on 10/03/2016
Application: In vitro bioactivity in cell culture
Reason for Rating: Very good recombinant protein.