Recombinant Human SIRP delta Fc Chimera Protein, CF

Catalog #: 10138-SB Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10138-SB-050

Recombinant Human SIRP delta Fc Chimera Protein Binding Activity

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human SIRP delta Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human SIRP delta Fc Chimera (Catalog # 10138-SB) is immobilized at 1 µg/mL (100 µL/well), recombinant human DIRAS2 binds with an ED₅₀ of 0.15-0.9 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human SIRP delta protein

Human SIRP delta (Phe30-Arg197) Accession # Q9H106	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q9H106

N-terminal Sequence
Analysis

Phe30

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

45 kDa

SDS-PAGE

50-60 kDa, under reducing conditions

Product Datasheets

10138-SB

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10138-SB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human SIRP delta Fc Chimera (Catalog # 10138-SB) is immobilized at 1 µg/mL, recombinant human DIRAS2 binds with an ED₅₀ of 0.15-0.9 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human SIRP delta Fc Chimera (Catalog # 10138-SB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-60 kDa and 100-120 kDa, respectively.

Reconstitution Calculator

Background: SIRP delta

SIRPD (Signal Regulatory Protein Delta), also know as Protein Tyrosine Phosphatase non-Receptor Type Substrate 1-l Like 2 (PTPNS1L2), is member of the signal regulatory proteins (SIRPS) family (1). SIRPD contains a 168 amino acid Ig-like domain that is characteristic of other SIRP family members (1). Unlike other members of SIRPS family, SIRPD lacks the transmemberane region, and is secreted (2). Murine homologs of SIRPD are not characterized. Expression sequence tag analysis suggests that SIRPD may be expressed in sperm cells and respiratory tissue (2). Using BioPlex 2.0 (Biophysical Interactions of ORFeome-derived complexes) high-throughput affinity purification–mass spectrometry (AP–MS) analysis to identify probable protein–protein interactions, several candidate SIRPD interactions were found including DIRAS2 (3). In-house testing indicates SIRPD can interact with DIRAS2.

References

Van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
Van Beek, E.M. et al. (2005) J Immunol. 175:7781.
Huttlin, E.L. et al. (2017) Nature 545:505.

Long Name

Signal-regulatory Protein delta

Entrez Gene IDs

128646 (Human)

Alternate Names

dJ576H24.4; Protein tyrosine phosphatase non-receptor type substrate 1-like 2; protein tyrosine phosphatase, non-receptor type substrate 1-like 2; PTPNS1L2; signal-regulatory protein delta; SIRP delta; SIRPD; SIRP-Delta