Recombinant Human SLITRK4 Protein, CF

Catalog #: 8945-SK Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8945-SK-050

R&D Systems Recombinant Proteins and Enzymes

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Recombinant Human SLITRK4 Protein, CF Summary

Product Specifications

Purity

>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 90 ng on a nitrocellulose-coated microplate.

Source

Mouse myeloma cell line, NS0-derived human SLITRK4 protein
Asp19-Pro616, with a C-terminal 6-His tag

Accession #

Q8IW52

N-terminal Sequence
Analysis

Asp19

Predicted Molecular Mass

68 kDa

SDS-PAGE

87-105 kDa, reducing conditions

Product Datasheets

8945-SK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8945-SK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: SLITRK4

SLITRK4 (Slit and Trk-like family member 4) is an approximately 90 kDa type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and (save for SLITRK1) a Trk-like cytoplasmic region (1). The extracellular domain (ECD) of mature human SLITRK4 contains 6 leucine rich repeats (LRR) followed by a C-terminal LRR domain, followed by 6 more LRR flanked by a pair of N- and C-terminal LRR domains (2, 3). Within the ECD, human SLITRK4 shares 98% amino acid sequence identity with mouse and rat SLITRK4. It is expressed in the testis and multiple regions of the brain, particularly the cerebral cortex, cerebellum, and hippocampus (4). SLITRK4 can suppress neurite outgrowth and promote the formation of excitatory presynaptic structures (3-5).

References