Recombinant Human SR-AI/MSR Protein, CF Summary
Product Specifications
Lys77-Leu451, with an N-terminal 9-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2708-MS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: SR-AI/MSR
The type I class A macrophage scavenger receptor (SR-AI; also MSR-AI) is a 70-80 kDa protein that belongs to an ever expanding family of transmembrane molecules collectively referred to as scavenger receptors (1 - 3). Receptors of this family contain characteristic extracellular domains and bind to a series of generally unrelated, but negatively-charged/polyanionic ligands (1, 3). Human SR-AI is a type II transmembrane glycoprotein that is 451 amino acids (aa) in length. It contains a 50 aa cytoplasmic tail, a 26 aa transmembrane segment and a 375 aa extracellular region (4, 5). The extracellular region contains four definitive domains, with a membrane proximal spacer of 33 aa, an alpha -helical coiled-coil domain of 163 aa, a collagen-like domain of 69 aa, and a cysteine-rich C-terminus of 110 aa (4, 6). The cysteine-rich domain (CRD) forms three intrachain disulfide bonds (7). The functional form of the molecule is a 220 - 230 kDa membrane-associated trimer that, in human, apparently has two disulfide bonded chains and a third noncovalently associated subunit (8, 9). Human extracellular region is 73% and 72% aa identical to bovine and mouse SR-AI extracellular region, respectively. The human gene for SR-A gives rise to three isoforms; the I isoform of 451 aa, the II isoform of 358 aa, and the III isoform of 388 aa (4, 5, 10). All are equal through the first 344 aa which includes the cytoplasmic tail through the collagenous domain. Isoform II (SR-AII) shows a severe truncation of the CRD, but is expressed on the cell surface. Isoform III (SR-AIII) has a modest truncation of the CRD, and cannot be expressed on the cell surface. Their functions are unknown. However, relative to SR-AI, SR-AII is known to show differential sensitivity to LPS and receptor binding to gram-negative bacteria,(9, 11) while SR-AIII is known to be a dominant-negative isoform (10). SR-AIII may achieve this by either heterotrimerizing with SR-AI, or simply eliminating the production of SR-AI mRNA.
- Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
- Linton, M.F. and S. Fazio (2001) Curr. Opin. Lipidol. 12:489.
- Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
- Matsumoto, A. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9133.
- Emi, M. et al. (1993) J. Biol. Chem. 268:2120.
- Naito, M. et al. (1992) Am. J. Pathol. 141:591.
- Resnick, D. et al. (1996) J. Biol. Chem. 271:26924.
- Ashkenas, J. et al. (1993) J. Lipid Res. 34:983.
- Penman, M. et al. (1991) J. Biol. Chem. 266:23985.
- Gough, P.J. et al. (1998) J. Lipid Res. 39:531.
- Peiser, L. et al. (2000) Inf. Immun. 68:1953.
Citations for Recombinant Human SR-AI/MSR Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Fibroblast-Derived STC-1 Modulates Tumor-Associated Macrophages and Lung Adenocarcinoma Development
Authors: T Kamata, TY So, Q Ahmed, S Giblett, B Patel, J Luo, R Reddel, C Pritchard
Cell Rep, 2020-06-23;31(12):107802.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Development -
Inhibition of antigen trafficking through scavenger receptor A.
Authors: Raycroft MT, Harvey BP, Bruck MJ, Mamula MJ
J. Biol. Chem., 2012-01-03;287(8):5310-6.
Applications: ELISA (Capture) -
Anti-class a scavenger receptor autoantibodies from systemic lupus erythematosus patients impair phagocytic clearance of apoptotic cells by macrophages in vitro.
Authors: Chen XW, Shen Y, Sun CY, Wu FX, Chen Y, Yang CD
Arthritis Res. Ther., 2011-01-31;13(1):R9.
Species: Human
Sample Types: Serum
Applications: ELISA (Capture)
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