Recombinant Human SREC-I/SCARF1 Fc Chimera Protein, CF
Recombinant Human SREC-I/SCARF1 Fc Chimera Protein, CF Summary
Product Specifications
| Human SREC-I (Ser20-Thr421) Accession # Q14162 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2409-SR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: SREC-I/SCARF1
The scavenger receptor (SR) family comprises a group of functionally defined membrane receptors that share a common ability to bind and internalize modified forms of low density lipoproteins (LDL) such as acetylated LDL (AcLDL) and oxidized LDL(OxLDL) (1-3). Family members are classified alphabetically. They play important roles in lipid metabolism, in host defence and in the regulation of acquired immunity (2, 4). Scavenger receptor expressed by endothelial cells-I (SREC-I) and SREC-II are two proteins that belong to the F type scavenger receptor group (SR-F1 and SR-F2). The full length cDNA of human SREC-I encodes an 830 amino acid (aa) type I transmembrane protein which contains a 19 aa signal peptide, a 402 aa extracellular region, a 21 aa transmembrane segment, and a 388 aa long cytoplasmic domain. The extracellular region contains ten EGF-like repeats (five of which fit the exact consensus sequence for an EGF-like domain) while the cytoplasmic domain is rich in serine and proline in the N-terminal half, and glycine in the C-terminal segment (5, 6). In addition to the full length form, four SREC-I isoforms exist. Two show insertions of a stop codon in EGF-like domain #8, resulting in mature soluble forms of 323 aa and 318 aa, respectively. A third isoform deletes part of domain #8 plus domains #9 and #10; it continues in-frame to generate a mature transmembrane protein of 725 aa. The last isoform shows only cytoplasmic splicing, with 72 aa substituted for the last 332 aa of the full length form. All three transmembrane forms bind acetylated LDL (6). Native SREC-I is approximately 150 kDa in size, and expressed by endothelial cells, macrophages and fetal neurons (7, 8). In the extracellular region human SREC-I is 76% aa identical to mouse SREC-I. The extracellular regions of human SREC-I and II are 53% aa identical.
- Horiuchi, S. et al. (2003) Amino Acids 25:283.
- Greaves, D.R. and S. Gordon (2005) J. Lipid Res. 46:11.
- Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
- Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
- Adachi, H. et al. (1997) J. Biol. Chem. 272:31217.
- Adachi, H. and M. Tsujimoto (2002) J. Biol. Chem. 277:24014.
- Shibata, M. et al. (2004) J. Biol. Chem. 279:40084.
- Tanura, Y. et al. (2004) J. Biol. Chem. 279:30938.
Citations for Recombinant Human SREC-I/SCARF1 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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SCARF-1 promotes adhesion of CD4+ T cells to human hepatic sinusoidal endothelium under conditions of shear stress
Authors: DA Patten, SK Kamarajah, JM Rose, J Tickle, EL Shepherd, DH Adams, CJ Weston, S Shetty
Sci Rep, 2017-12-14;7(1):17600.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Oxidation by neutrophils-derived HOCl increases immunogenicity of proteins by converting them into ligands of several endocytic receptors involved in antigen uptake by dendritic cells and macrophages.
Authors: Biedron R, Konopinski M, Marcinkiewicz J, Jozefowski S
PLoS ONE, 2015-04-07;10(4):e0123293.
Species: Mouse
Sample Types: Protein
Applications: Binding Assay -
A nasal epithelial receptor for Staphylococcus aureus WTA governs adhesion to epithelial cells and modulates nasal colonization.
Authors: Baur S, Rautenberg M, Faulstich M, Grau T, Severin Y, Unger C, Hoffmann W, Rudel T, Autenrieth I, Weidenmaier C
PLoS Pathog, 2014-05-01;10(5):e1004089.
Applications: Bioassay -
Oligo-guanosine nucleotide induces neuropilin-1 internalization in endothelial cells and inhibits angiogenesis.
Authors: Narazaki M, Segarra M, Hou X, Tanaka T, Li X, Tosato G
Blood, 2010-07-06;116(16):3099-107.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay -
Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A.
Authors: Narazaki M, Segarra M, Tosato G
Blood, 2008-02-13;111(8):4126-36.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay
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