Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein, CF

Catalog #: AVI11066 Datasheet / COA / SDS

Biotinylated

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AVI11066-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

When Recombinant Human IL-33 (Catalog # 3625-IL) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human ST2/IL-33R Fc Chimera Avi-tag (Catalog # AVI11066) binds with an ED₅₀ of 90.0-720 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human ST2/IL-33R protein

Human ST-2 (Lys19-Ser328) Accession # Q01638.4	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

Q01638.4

N-terminal Sequence
Analysis

Lys19

Structure / Form

Covalent homo-dimer

Biotinylated via Avi-tag

Predicted Molecular Mass

63 kDa

SDS-PAGE

90-105 kDa, under reducing conditions.

Product Datasheets

AVI11066

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11066

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human IL-33 (3625-IL) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein (Catalog # AVI11066) binds with an ED₅₀ of 90.0-720 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human ST2/IL-33R Fc Chimera Avi-tag Protein (Catalog # AVI11066) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 90-105 kDa and 180-210 kDa, respectively.

Reconstitution Calculator

Background: ST2/IL-33R

Serum stimulation-2 (ST2), also known as Interleukin receptor like-1 (IL1RL1) and T1, is a member of the Interleukin-1 receptor superfamily family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of an extracellular domain (ECD) with three Ig-like domains, a transmembrane segment, and a cytoplasmic domain with an intracellular Toll/interleukin-1 receptor (TIR) domain (3, 4). Within the ECD, human ST2 shares 68% and 64% amino acid sequence identity with mouse and rat ST2, respectively. Alternate splicing of human ST2 generates a soluble isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5-8). It binds IL33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL1R AcP, a shared signaling subunit that also associates with IL1RI and IL1R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL1RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12-14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks IL33 signaling by full-length ST2 (10, 13‑15). Our Avi-tag Biotinylated ST2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
Tominaga, S. et al. (1992) Biochim. Biophys. Acta. 1171:215.
Li, H. et al. (2000) Genomics 67:284.
Schmitz, J. et al. (2005) Immunity 23:479.
Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
Weinberg, E.O. et al. (2002) Circulation 106:2961.
Sanada S. et al. (2007) J. Clin. Invest. 117:1538.
Palmer, G. et al. (2008) Cytokine 42:358.
Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
Verri, Jr. W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.

Long Name

Interleukin 33 Receptor

Entrez Gene IDs

9173 (Human); 17082 (Mouse)

Alternate Names

DER4; DER4ST2growth stimulation-expressed; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33 R; IL33R; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; interleukin-1 receptor-like 1; Ly84; MGC32623; Protein ST2; ST2; ST2L; ST2V; T1; T1homolog of mouse growth stimulation-expressed