Recombinant Human Stabilin-2 His-tag Protein, CF

Catalog #: 11086-S2 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11086-S2-050

Recombinant Human Stabilin‑2 His-tag Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Stabilin-2 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Stabilin-2 His-tag Protein is immobilized at 2.00 μg/mL (100 μL/well), Biotinylated Hyaluronan binds with an ED₅₀ of 1.50-7.50 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human Stabilin-2 protein
Ser20-Thr2458, with a C-terminal 6-His tag

Accession #

Q8WWQ8.3

N-terminal Sequence
Analysis

Ser20

Predicted Molecular Mass

265 kDa

SDS-PAGE

260-300 kDa, under reducing conditions.

Product Datasheets

11086-S2

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11086-S2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human Stabilin-2 His-tag Protein (Catalog # 11086-S2) is immobilized at 2.00 μg/mL (100 μL/well), Biotinylated Hyaluronan binds with an ED₅₀ of 1.50-7.50 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human Stabilin‑2 His-tag Protein (Catalog # 11086-S2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 260-300 kDa.

Reconstitution Calculator

Background: Stabilin-2

Stabilin-2, also known as HARE (Hyaluronic Acid Receptor for Endocytosis) and FEEL2 (Fasciclin, EGF-like, laminin type EGF-like, and Link domain containing scavenger receptor 2), is a type I transmembrane multidomain protein that is the closest homolog of stabilin1. It is a scavenger receptor that is expressed on sinusoidal endothelial cells of liver, spleen, and lymph node (1, 2). Its 2439 amino acid (aa) extracellular domain contains seven fasciclin domains, multiple EGF-like and laminin type EGF-like domains, and a link domain related to molecules of the TSG6 superfamily (3). The 72 aa cytoplasmic tail of Stabilin2 contains a motif that allows the AP2 classical cargo adaptor to direct cargo into clathrincoated pits (4). Stabilin2 also mediates the endocytosis of chondroitin and chondroitin sulfate, advanced glycosylation endproduct (AGE), collagen Nterminal propeptides and acetylated LDL (4-7). Stabilin-2 has been found to regulate the cancer progression, and high expression of Stabilin-2 was reported to predict poor tumor prognosis in NSCLC (8).

References

Politz, O. et al. (2002) Biochem. J. 362:155.
Harris, E.N. and F. Cabral (2019) Biomolecules 9:273.
Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
Hansen, B. et al. (2005) Exp. Cell Res. 303:160.
Harris, E.N. et al. (2004) J. Biol. Chem. 279:36201.
Harris, E.N. et al. (2007) J. Biol. Chem. 282:2785.
Tamura, Y. et al. (2003) J. Biol. Chem. 278:12613.
Yong, J. et al. (2021) Bioengineered 12:3426.

Entrez Gene IDs

55576 (Human); 192188 (Mouse); 282580 (Rat)

Alternate Names

CD44-like precursor FELL; DKFZP434E0321; FAS1 EGF-like and X-link domain containing adhesion molecule-2; FAS1 EGF-like and X-link domain-containing adhesion molecule 2; fasciclin egf-like, laminin-type egf-like, and link domain-containing scavengerreceptor-2; Fasciclin, EGF-like, laminin-type EGF-like and link domain-containing scavengerreceptor 2; FEEL2; FEEL-2; FELE-2; FELL; FELL-2; FEX2; HARE; HAREFELL2; hepatic hyaluronan clearance receptor; Hyaluronan receptor for endocytosis; hyaluronic acid receptor for endocytosis; STAB2; STAB-2; stabilin 2; Stabilin2; Stabilin-2