Recombinant Human Stanniocalcin 2/STC-2 Protein, CF

Catalog #: 9405-SO Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9405-SO-050

Recombinant Human Stanniocalcin 2/STC-2 Protein Bioactivity

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All Stanniocalcin 2/STC-2 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Stanniocalcin 2/STC-2 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 60-360 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

Source

Mouse myeloma cell line, NS0-derived human Stanniocalcin 2/STC-2 protein
Thr25-Arg302, with a C-terminal 6-His tag

Accession #

O76061

N-terminal Sequence
Analysis

Thr25

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

31 kDa

SDS-PAGE

34-43 kDa, reducing conditions

Product Datasheets

9405-SO

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9405-SO

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human Stanniocalcin 2/STC-2 (Catalog # 9405-SO) inhibits Recombinant Mouse Wnt-3a induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED₅₀ for this effect is 60-360 ng/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

Reconstitution Calculator

Background: Stanniocalcin 2/STC-2

Stanniocalcin 2 (STC-2) is a secreted disulfide-linked homodimeric glycoprotein hormone that is related to the STC protein first discovered from corpuscles of stannius in fish (1). The STC-2 has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues (2). Its C-terminus contains a cluster of histidine residues which may interact with metal ions (1). STC-2 is expressed in a wide variety of tissues. In the ovary, STC-2 has been shown to be a paracrine hormone that regulates granulosa cell function (1). The amino acid sequence of human mature STC-2 is 36% identical to that of human STC-1. It is also 99% and 88% identical to that of monkey and mouse STC-2, respectively (3). STC-2 enhances mesenchymal stem cell survival (4), promotes cell proliferation in cervical cancer (5), suppresses breast cancer cell migration and invasion (6), promotes osteoblast differentiation (7), and inhibits longitudinal bone growth directly at the growth plate (8). It is also a biomarker for cervical and lung cancers (9, 10),

References