Recombinant Human TAPBPR Fc Chimera Protein, CF

Catalog #: 11171-TP Datasheet / COA / SDS

(CHO-expressed)

Catalog #	Availability	Size / Price	Qty
11171-TP-050

Recombinant Human TAPBPR Fc Chimera Protein Bioactivity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TAPBPR Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.50 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 0.500‑10.0 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human TAPBPR protein

Human TAPBPR (Ala19-Arg404) Accession # Q9BX59.2	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q9BX59.2

N-terminal Sequence
Analysis

Ala19

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

68 kDa

SDS-PAGE

75-90 kDa, under reducing conditions.

Product Datasheets

11171-TP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11171-TP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion by human T cells. The ED₅₀ for this effect 0.500‑10.0 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human TAPBPR Fc Chimera Protein (Catalog # 11171-TP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-90 kDa and 150-180 kDa, respectively.

Reconstitution Calculator

Background: TAPBPR

TAP-binding protein-like (TAPBPL), also known as TAP binding protein-related (TAPBPR) and Tapasin-related protein (TAPASINR) is a transmembrane protein of the Immunoglobulin (Ig) superfamily (1, 2). TAPBPR was originally isolated as a homologue to TAPASIN but more recently was identified as a novel B7 family-related molecule since it shares sequence, structural, and functional similarities to B7 family members (3). Mature human TAPBPR consists of a lumenal domain containing an IgV and IgC domain, a transmembrane domain, and a cytoplasmic tail which lacks an ER retention motif. Within the lumenal domain, mature human TAPBPR shares 70% and 71% amino acid sequence identity with mouse and rat TAPBPR, respectively. Multiple alternatively spliced TAPBPR isoforms are known to exist with unique properties (4).TAPBPR is widely expressed and, similar to TAPASIN, functions as a both a chaperone protein and peptide editor of MHC class I, but in a peptide‑loading complex (PLC) independent manner (5, 6). TAPBPR decreases the rate at which MHC class I molecules mature through the secretory pathway, a role which could be important for peptide selection by MHC class I molecules (7). TAPBPR is also expressed on the surface of T cells and antigen-presenting cells (APCs) and plays an inhibitory role in the proliferation and activation of T cells (4). TAPBPR can be expressed on various cancer cells like leukemia and has the potential to be used in the treatment of autoimmune diseases and transplant rejection, as well as cancer (4).

References

Hermann, C. et al. (2015) Tissue antigens 85(3):155.
Teng, M. et al. (2002) European Journal of Immunology 32:1059.
Lin, Y. et al. (2021). EMBO Mol Med. 13(5):13404.
Porter, K.M. et al. (2014) Immunology 142:289.
Margulies, D. et al. (2020) Current Opinion in Immunology 64:71.
Boyle, L.H. et al. (2013) PNAS 110:3465.
Hermann, C. et al. (2013) Journal of Immunology 191(11):5743.

Long Name

Tapasin Related Protein

Entrez Gene IDs

55080 (Human); 213233 (Mouse); 297602 (Rat)

Alternate Names

FLJ10143; TAP binding protein-like; Tapasin Related Protein/TAPBPR; Tapasin-like; TAPASIN-R; tapasin-related protein; TAP-binding protein-like; TAP-binding protein-related protein; TAPBPL; TAPBPR; TAPBP-RTAP binding protein related; TAPBPRtapasin-like