Recombinant Human TIM-1/KIM-1/HAVCR (275 aa) Fc Protein, CF

Recombinant Human TIM-1/KIM-1/HAVCR (275 aa) Fc Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background:

T cell-immunoglobulin and mucin domain 1 (TIM-1), also known as KIM1 and HAVcr1, type I transmembrane glycoprotein in the TIM subfamily of immunoglobulin (Ig) superfamily molecules. In human, the TIM family is comprised of 3 family members and is involved in the regulation of Th1 and Th2‑cell‑mediated immune responses (1). Mature TIM-1 consists of an extracellular domain (ECD) with one V‑type Ig‑like domain and a mucin domain with O- and N-linked carbohydrates, a transmembrane segment, and a cytoplasmic signaling domain. Within the ECD, human TIM-1 shares 39% amino acid sequence identity with mouse TIM-1. Multiple TIM-1 variants can be produced either through polymorphisms or alternative splicing primarily resulting in deletions in the mucin domain (2). A soluble form of TIM-1, detectable in the urine and in circulation, can be generated through ectodomain cleavage mediated by MMP3 (3, 4). TIM-1 is expressed on splenic B cells, IL-10+ regulatory B cells, CD4+ T cells, mast cells, dendritic cells, kidney epithelium and a broad range of mucosal epithelium (5-7). It is upregulated on activated Th2 cells, after dendritic cell maturation, and on kidney tubular epithelial cells after injury (8-10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (5, 11). TIM-1 serves as a receptor for phosphatidylserine, and its interaction with LMIR5 enables TIM-1 to mediate the phagocytosis of apoptotic cells (12). TIM-1 also serves as a cellular entry receptor for various viruses, including hepatitis A virus, Ebolavirus, Marburgvirus and has been indicated as a possible receptor for SARS-CoV-2 (13-15).