Recombinant Human TMED1 Fc Chimera Protein, CF

Catalog #: 2243-TM Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
2243-TM-050

Recombinant Human TMED1 Fc Chimera Protein Binding Activity

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TMED1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human TMED1 Fc Chimera is coated at 0.5 µg/mL, 100 μL/well, Recombinant Human ST2/IL‑1 R4 Fc Chimera (Catalog # 523-ST) binds with an ED₅₀ of 0.6-3.6 μg/mL

Source

Human embryonic kidney cell, HEK293-derived human TMED1 protein

Human TMED1 (Ala24-Asn194) Accession # Q13445	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q13445

N-terminal Sequence
Analysis

Ala24

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

46 kDa

SDS-PAGE

56-62 kDa, reducing conditions

Product Datasheets

2243-TM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2243-TM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant TMED1 Fc Chimera (Catalog # 2243-TM) is coated at 0.5 µg/mL, Recombinant Human ST2/IL-1 R4 Fc Chimera (Catalog # 523-ST) binds with an ED₅₀ of 0.6-3.6 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human TMED1 Fc was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 56-62 kDa and 110-120 kDa, respectively.

Reconstitution Calculator

Background: TMED1

TMED1 (Transmembrane Emp24 domain-containing protein 1) is a member of the TMED family of proteins (gene name TMED1). The TMED family of proteins are localized to membranes of the early secretory pathway, including the endoplasmic reticulum and Golgi, and function in vesicular protein trafficking (1, 2). TMED1 is a 59 kDa monomer and has been reported to exist as homodimer (3). TMED1 is composed of a 23 amino acid (aa) signal sequence, a 171 aa extra cellular domain, a 21 aa transmembrane domain, and a 12 aa cytoplasmic domain. The extracellular domain contains an 83 aa GOLD (Golgi Dynamics) domain, and COPI and COPII binding motifs are found in the cytoplasmic domain (1-3, 5). Human TMED1 shares 97% sequence identity with mouse, bovine, and rat homologs within the 171 aa extracellular domain. The beta -strand-rich GOLD domain has been specifically identified to be involved in intracellular protein trafficking (1, 4, 5). TMED1 is important in regulating innate immune signaling through its interaction with ST2L. Specifically, the GOLD domain in TMED1 interacts with the TIR domain of ST2L, a receptor for IL‑33 (1). This interaction promotes ST2L association with IL-33, allowing downstream signaling cascade activating MAP kinases, p38, and JNK (1, 6). Studies have shown knockdown of TMED-1 in HUVECs impairs the IL-33 induced response resulting in reduction of IL-6 and IL-8 productions (1).

References

Connolly, D. et al. (2013) J Biol Chem. 288:5616.
Gour, N. and Lajoie, S. (2018) Curr Allergy Ashma Rep. 16:65.
Jenne, N. (2002) J Biol Chem. 277:46504.
Anantharaman, V. and Aravind, L. (2002) Genome Biol. 3:research0023
Gomez-Navarro, N. and Miller, E. (2016) J Cell Biol. 215:769.
Hardman, C. and Ogg, G. (2016). Curr Opin Immunol. 42:16.

Entrez Gene IDs

11018 (Human); 17083 (Mouse); 315461 (Rat)

Alternate Names

IL1RL1-Binding Protein; Il1rl1l; IL1RL1LG; IL-1RL1LG; IL1RL1LGIL1RL1-binding protein; Interleukin 1 Receptor-Like 1 Ligand; Interleukin-1 receptor-like 1 ligand; Ly84l; MGC1270; P24g1; P24gamma1; Putative T1/ST2 receptor-binding protein; ST2L; T1/ST2 receptor binding protein; TMED1; Tp24; transmembrane emp24 domain containing 1; transmembrane emp24 domain-containing protein 1; transmembrane emp24 protein transport domain containing 1